Fluorescence microscopical studies on noradrenaline in the peripheral blood vessels of spontaneouly hypertensive rats.
Fluorescence microscopical studies on noradrenaline in the peripheral blood vessels of spontaneouly hypertensive rats.
复制标题
自发性高血压大鼠外周血管去甲肾上腺素的荧光显微镜研究。
DOI：
10.1253/jcj.32.1391
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发表时间：
1968
期刊：
影响因子：
--
通讯作者：
K. Okamoto
中科院分区：
文献类型：
--
作者：
H. Haebara;K. Ichijima;T. Motoyoshi;K. Okamoto
用高特异性和高灵敏度的荧光法对自发性高血压大鼠(Okamoto and Aoki，SHR)外周血管的肾上腺素能神经支配进行了研究，并与正常血压大鼠作了比较。1)自发性高血压大鼠大脑前动脉和大脑中动脉直径>1.5 0/h时，肾上腺素能神经末梢的荧光强度略有增强，但直径小于15 0μ的自发性高血压大鼠肾上腺素能神经末梢的荧光强度与对照组无明显差异。2)其他大脑主要动脉及其分支的肾上腺素能神经分布相似，荧光强度无明显差异。大脑主要动脉向远端分支越多，荧光分布越稀疏，除漏斗部位外，脑内动脉无特异性肾上腺素能荧光。3)自发性高血压大鼠脉络膜动脉直径50~15μ处可见肾上腺素能荧光明显增强，神经末梢静脉曲张的颗粒或云团较对照组明显增大。4)自发性高血压大鼠肠小动脉直径100~40μ的扩张和收缩部分均可见荧光增强，荧光颗粒或小云团较对照组大，强度较高。5)自发性高血压大鼠冠状动脉小动脉肾上腺素能荧光丰富且增多。6)在高血压的三个阶段，即高血压前期、高血压初期和高血压晚期，均可观察到这些增强的荧光。7)三种定量方法检测到的自发性高血压大鼠的增强荧光数量均有明显增加。上述结果提示，去甲肾上腺素作为外周血管的神经递质，可能促进血管功能，在自发性高血压的发病机制中起重要作用。
Adrenergic innervations of the peripheral blood vessels of the Spontaneously Hypertensive Rats (OKAMOTO and AOKI) (SHR) were studied by the highly specific and sensitive fluorescence method, in comparison with those of normotensive control rats, and the following results were obtained. 1) Anterior cerebral and middle cerebral arteries over 1 50/h in diameter showed slightly augmented fluorescence in adrenergic nerve terminals in the SHR, but in those less than 150μ in diameter there was no obvious difference in fluorescence between the SHR and controls. 2) Other main cerebral arteries and their branches exhibited the similar distribution of adrenergic innervations and apparent difference in fluorescence was not seen. The more the main cerebral arteries branched out to the distal the more scanty became the tendency in fluorescence distributions, and there was no specific adrenergic fluorescence of the arteries in the intracerebral portion except in the infundibular area. 3) Marked augmented adrenergic fluorescences were seen in the choroidal arteries 50-15μ in diameter in the SHR and granules or cloudlets, called the varicosities of the nerve terminals, were larger than those of controls. 4) The dilated and constricted portions of the intestinal small arteries 100-40μ in diameter in the SHR always revealed augmented fluorescence and granules or cloudlets of fluorescence were larger and more intense than those of controls. 5) The adrenergic fluorescence of small coronary arteries in the SHR was numerous and increased in number. 6) These augmented fluorescences were observed throughout the three stages of hypertension; that is, the prehypertensive, initial, and advanced stages, respectively. 7) The obtained augmented fluorescence in the SHR showed a clear increase in quantity examined by three quantitative methods. 8) Above-mentioned findings suggested that noradrenaline, as the neurotransmitter, of the peripheral blood vessels may accelerate vascular function and play some important roles in the pathogenesis of the SHR.