Effects of bile acids on colon carcinogenesis in rats treated with carcinogens.
Effects of bile acids on colon carcinogenesis in rats treated with carcinogens.
复制标题
胆汁酸对用致癌物治疗的大鼠结肠癌发生的影响。
作者：
B. Cohen;R. Raicht
在化学诱导的结肠癌大鼠模型中，研究了初级胆汁酸作为可能的结肠肿瘤促进剂或抑制剂。胆酸喂养增加了患有肿瘤的动物数量、每只动物的肿瘤数量以及每只荷瘤动物的肿瘤数量。肿瘤增强归因于脱氧胆酸，胆酸的细菌代谢物。当给我们的模型中的大鼠喂食鹅去氧胆酸时，肿瘤发生率增加，但每只动物的肿瘤数量和每只荷瘤动物的肿瘤数量与对照组相似。用鹅去氧胆酸获得的不同粪便胆汁酸模式可能是肿瘤发生率差异的原因。表征和鉴定粪便中所有甾体成分的方法需要提取、薄层色谱法、气液色谱法和气液色谱-质谱法。较新的技术包括 LH-20 色谱法（用于硫酸化类固醇）和高压液相色谱法。
Primary bile acids were studied as possible colon tumor promoters or inhibitors in a rat model of chemically induced colon cancer. Cholic acid feeding increased the number of animals with tumors, the number of tumors per animal, and the number of tumors per tumor-bearing animal. Tumor enhancement was attributed to deoxycholic acid, the bacterial metabolite of cholic acid. When chenodeoxycholic acid was fed to the rats in our model, tumor incidence was increased, but the number of tumors per animal and the number of tumors per tumor-bearing animal were similar to controls. The different fecal bile acid pattern obtained with chenodeoxycholic acid may be responsible for the differences in tumor incidence. The methodology to characterize and identify all steroidal components of the feces requires extraction, thin-layer chromatography, gas-liquid chromatography, and gas-liquid chromatography-mass spectrometry. Newer techniques include LH-20 chromatography (for sulfated steroids) and high-pressure liquid chromatography.