A role for RIC-8 (Synembryn) and GOA-1 (G(o)alpha) in regulating a subset of centrosome movements during early embryogenesis in Caenorhabditis elegans.
A role for RIC-8 (Synembryn) and GOA-1 (G(o)alpha) in regulating a subset of centrosome movements during early embryogenesis in Caenorhabditis elegans.
复制标题
RIC-8 (Synembryn) 和 GOA-1 (G(o)alpha) 在秀丽隐杆线虫早期胚胎发生过程中调节中心体运动子集的作用。
DOI：
10.1093/genetics/156.4.1649
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发表时间：
2000
期刊：
影响因子：
3.3
通讯作者：
Rand,JB
中科院分区：
文献类型：
--
作者：
Miller,KG;Rand,JB
RIC-8 (synembryn) and GOA-1 (Goα) are key components of a signaling network that regulates neurotransmitter secretion inCaenorhabditis elegans.Here we show thatric-8andgoa-1reduction of function mutants exhibit partial embryonic lethality. Through Nomarski analysis we show thatgoa-1andric-8mutant embryos exhibit defects in multiple events that involve centrosomes, including one-cell posterior centrosome rocking, P1centrosome flattening, mitotic spindle alignment, and nuclear migration. Inric-8reduction of function backgrounds, the embryonic lethality, spindle misalignments and delayed nuclear migration are strongly enhanced by a 50% reduction in maternalgoa-1gene dosage. Several other microfilament- and microtubule-mediated events, as well as overall embryonic polarity, appear unperturbed in the mutants. In addition, our results suggest that RIC-8 and GOA-1 do not have roles in centrosome replication, in the diametric movements of daughter centrosomes along the nuclear membrane, or in the extension of microtubules from centrosomes. Through immunostaining we show that GOA-1 (Goα) localizes to cell cortices as well as near centrosomes. Our results demonstrate that two components of a neuronal signal transduction pathway also play a role in centrosome movements during early embryogenesis.
