The antivascular action of physiotherapy ultrasound on murine tumors
The antivascular action of physiotherapy ultrasound on murine tumors
复制标题
DOI：
10.1016/j.ultrasmedbio.2005.06.008
复制
发表时间：
2005-10-01
影响因子：
2.9
通讯作者：
Sehgal, CM
中科院分区：
文献类型：
--
作者：
Wood, AKW;Ansaloni, S;Sehgal, CM
本研究旨在确定物理治疗超声（US）是否影响肿瘤中脆弱和渗漏的血管生成血管。22只C3HV/HeN小鼠皮下黑色素瘤（K1735(22)）被连续1 mhz低强度（空间-平均-时间-平均= 2.28 W cm(-2)）照射（1、2或3分钟），物理治疗US。进行对比度增强（0.1 mL option (R)）功率多普勒超声观察，并对图像进行直方图分析。超声前，除了7%的肿瘤外，其余肿瘤都进行了灌注。接受3分钟治疗的肿瘤无血管面积增加至82% （p < 0.001）。线性回归分析显示，每分钟照射可使肿瘤血管密度降低25%；抗血管活性持续24小时。组织学显示血管壁破坏和血管充血和血栓形成区域的肿瘤细胞死亡。物理治疗美国特别针对血管结构，对肿瘤细胞的影响似乎是继发于由此产生的缺血。
This study was aimed at determining if physiotherapy ultrasound (US) affected the fragile and leaky angiogenic blood vessels in a tumor. In 22 C3HV/HeN mice, a subcutaneous melanoma (K1735(22)) was insonated (1, 2 or 3 min) with continuous 1-MHz low-intensity (spatial-average temporal-average = 2.28 W cm(-2)), physiotherapy US. Contrast-enhanced (0.1 mL Optison (R)) power Doppler US observations were made and histogram analyses of the images were performed. Before insonation, all but 7% of the tumor was perfused. The avascular area in tumors receiving 3-min treatment increased to 82% (p < 0.001). A linear regression analysis showed that each min of insonation led to a 25% reduction in tumor vascularity; the antivascular activity persisted for 24 h. Histology demonstrated disruption of vascular walls and tumor cell death in areas of vascular congestion and thrombosis. Physiotherapy US particularly targeted the vascular structures, and the effects on tumor cells appeared to be secondary to the resultant ischemia.