Arginine affects growth and integrity of grass carp enterocytes by regulating TOR signaling pathway and tight junction proteins
Arginine affects growth and integrity of grass carp enterocytes by regulating TOR signaling pathway and tight junction proteins
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DOI：
10.1007/s10695-019-00613-w
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发表时间：
2019-04-01
影响因子：
2.9
通讯作者：
Hu, Pengcheng
中科院分区：
文献类型：
--
作者：
Chen, Jiaojiao;Zhang, Dianfu;Hu, Pengcheng
饲料中添加精氨酸(Arg)能改善草鱼肠道结构和吸收，但其改善肠道形态的作用机制尚不清楚。本研究旨在阐明精氨酸对草鱼肠道上皮细胞产生积极作用的可能机制。采用体外原代培养模型，观察不同浓度精氨酸(0、0.1、0.2、0.5、1.0、2.0 mmol/L)对肠上皮细胞生长、细胞活力、TOR信号通路及紧密连接蛋白表达的影响。结果表明，0.5 mmol/L精氨酸能增加细胞数，降低培养上清液中乳酸脱氢酶和肌酸激酶活性(P
Dietary arginine (Arg) could improve the intestinal structure and absorption of grass carp (Ctenopharyngodon idellus); however, the mechanism of Arg on intestinal morphology improvement was unclear. The present study aimed to explain the possible mechanism of the positive effect of Arg on intestinal epithelial cells of grass carp. An in vitro study was conducted through a primary culture model to assess the growth, cell viability, mRNA expressions of TOR signal pathway, and tight junction proteins of enterocytes after culture in the medium with 6 levels of Arg (0, 0.1, 0.2, 0.5, 1.0, and 2.0mmol/L). The results showed that 0.5mmol/L Arg improved the cell number and decreased the lactate dehydrogenase and creatine kinase activities in culture medium (P