STATISTICAL-ANALYSIS OF DNA FINGERPRINT DATA FOR ORDERED CLONE PHYSICAL MAPPING OF HUMAN-CHROMOSOMES
STATISTICAL-ANALYSIS OF DNA FINGERPRINT DATA FOR ORDERED CLONE PHYSICAL MAPPING OF HUMAN-CHROMOSOMES
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DOI：
10.1016/s0092-8240(05)80411-2
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发表时间：
1991-01-01
影响因子：
3.5
通讯作者：
TORNEY, DC
中科院分区：
文献类型：
--
作者：
BALDING, DJ;TORNEY, DC
提出了一种用于分析DNA指纹数据的统计框架，该框架的目的是构建染色体的有序克隆物理图谱。指纹数据由限制性消化片段的长度和杂交状态组成，本文提出了一种解决决定两个随机选择的克隆是否重叠的基本问题的方法。重叠概率的计算使用贝叶斯规则与适当的统计描述的染色体和实验程序。分析是灵活的，允许各种假设来解释实验误差和困难，例如未观察到的碎片。这里描述的方法提供了一个基础，用于预测的进展速度的实验方案，因此比较替代方案。它很容易推广到具有广泛可能数据的相关问题。给出了洛斯阿拉莫斯国家实验室目前在人类16号染色体上使用的克隆作图方案的结果（Stallings等人，1990，Proc. natl. Acad. Sci.美国，87，6218-6222）。
A statistical framework is proposed for analysing DNA fingerprint data from experiments aimed at constructing ordered clone physical maps of chromosomes. The fingerprint data consists of the lengths and hybridization states of restriction digest fragments and the paper develops a solution to the fundamental problem of deciding whether or not two randomly selected clones overlap. Overlap probabilities are calculated using Bayes' rule together with appropriate statistical descriptions of the chromosome and experimental procedure. The analysis is flexible, allowing a variety of assumptions to account for experimental errors and difficulties, such as unobserved fragments. The approach described here provides a basis for predicting the rate of progress of an experimental protocol and hence for comparing alternate protocols. It is readily generalized to related problems with a wide range of possible data. Results are presented for the clone mapping protocol currently being employed at Los Alamos National Laboratory on human chromosome 16 (Stallings et al., 1990, Proc. natl. Acad. Sci. U.S.A., 87, 6218-6222).