Identification and immunohistochemical characterization of a mucin-like glycoprotein expressed in early stage breast carcinoma
Identification and immunohistochemical characterization of a mucin-like glycoprotein expressed in early stage breast carcinoma
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DOI：
10.1159/000068566
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发表时间：
2002-09-01
期刊：
影响因子：
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通讯作者：
Stroupe, S
中科院分区：
文献类型：
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作者：
Colpitts, TL;Billing, P;Stroupe, S
在这份报告中，我们描述了从Incell基因组LifeSeq((R))表达序列标签数据库中鉴定的一个cDNA序列，BS106。多组织mRNA表达阵列、Northern杂交和RT-PCR分析表明，BS106在乳腺、唾液和前列腺癌中表达，但在其他类型的组织中不表达。90%的乳腺组织中检测到BS106基因的表达。该基因编码一个90个氨基酸的蛋白质，其特征是一种小的粘蛋白样蛋白，基于氨基酸组成、广泛的O-连接糖基化和表达谱。在人胚胎肾293细胞中表达BS106蛋白，并从培养上清液中纯化BS106蛋白的分泌产物。制备单抗，用于早期乳腺癌的免疫组织化学分析。在绝大多数(70-100%)的癌组织中检测到BS106蛋白，在所分析的22个样本中，大约有30%的组织中BS106蛋白过表达。BS106蛋白在膀胱癌、结肠癌、子宫内膜癌、胃癌、鳞状细胞肺癌、肺腺癌、卵巢癌、胰腺癌、前列腺癌等实体瘤中均未检测到。版权所有(C)2002 S.Karger AG，巴塞尔。
In this report we describe a cDNA sequence, BS106, identified from Incyte Genomics LifeSeq((R)) Expressed Sequence Tag database. A multi-tissue mRNA expression array, northern blots, and RT-PCR assays demonstrate the expression of BS106 in mammary, salivary and prostate glands, but not in other tissue types. BS106 mRNA was detected in 90% of the breast tissues examined. The cDNA encodes a 90-amino acid protein characterized as a small, mucin-like protein based on amino acid composition, extensive O-linked glycosylation, and expression profile. BS106 protein was recombinantly expressed in human embryonic kidney 293 cells and the secreted product was purified from the culture media. Monoclonal antibodies were prepared and used for immunohistochemical analysis of early stage breast cancer. BS106 protein was detected in the vast majority of carcinomas (70-100%) and overexpressed in approximately 30% of the 22 specimens analyzed. BS106 protein was not detected in other solid tumor types including bladder carcinoma, colon carcinoma, endometrial carcinoma, gastric carcinoma, squamous cell lung carcinoma, adenocarcinoma of the lung, ovarian carcinoma, pancreatic and prostatic carcinoma. Copyright (C) 2002 S. Karger AG, Basel.