Cell killing by simian virus 40: evaluation of the role of extracellular calcium.
Cell killing by simian virus 40: evaluation of the role of extracellular calcium.
复制标题
猿猴病毒 40 的细胞杀伤：细胞外钙作用的评估。
DOI：
10.1016/0042-6822(82)90432-9
复制
发表时间：
1982
期刊：
影响因子：
3.7
通讯作者：
Norkin,LC
中科院分区：
文献类型：
--
作者：
Norkin,LC
Previous results of other investigators demonstrated that the killing of rat liver hepatocytes by each of a number of different membrane-active agents showed an absolute requirement for calcium in the medium. Earlier, we reported that altered membrane permeability is a major determinant of lethal cell injury caused by lytic simian virus 40 (SV40) infection. Results of the present study show that killing of CV-1 green monkey kidney cells by SV40, as determined by trypan blue staining, followed a similar time course and occurred to the same extent in calcium-free as in calcium-containing medium. The lethal consequence of calcium influx into CV-1 cells was nevertheless demonstrated by the cytotoxic action of the calcium-specific ionophore A23187, which rapidly killed CV1 cells in calcium-containing but not in calcium-free medium. It is concluded that whereas calcium influx is very cytotoxic to cells which are susceptible to lytic SV40 infection, calcium influx is not required to couple SV40-induced cell membrane damage to consequent cell death.
