UTILIZATION OF CARBON-1 COMPOUNDS BY PLANTS .I. METABOLISM OF METHANOL-C14 + ITS ROLE IN AMINO ACID BIOSYNTHESIS
UTILIZATION OF CARBON-1 COMPOUNDS BY PLANTS .I. METABOLISM OF METHANOL-C14 + ITS ROLE IN AMINO ACID BIOSYNTHESIS
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DOI：
10.1139/o64-190
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发表时间：
1964-01-01
期刊：
影响因子：
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通讯作者：
COSSINS, EA
中科院分区：
文献类型：
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作者：
COSSINS, EA
甲醇-C14在胡萝卜组织切片、豌豆子叶、大豆子叶、蓖麻籽胚乳、甜菜贮藏组织和甜菜成熟叶片中代谢迅速。除了甜菜的贮藏组织外，二氧化碳是甲醇代谢的主要产物。在所有组织中，有机酸、糖、氨基酸和不溶性残留物中也含有甲醇碳。丝氨酸、蛋氨酸、蛋氨酸砜和蛋氨酸亚砜是分离出的氨基酸组分中的重要标记组分。对代谢甲醇-C14的胡萝卜组织产生的丝氨酸-C14的降解表明，大部分标记存在于3位。这些结果被解释为表明甲醇可以作为碳-1单元的前体，这些碳-1单元将用于导致丝氨酸和蛋氨酸生物合成的转甲基化反应。此外，甲醇可以被这些组织氧化成二氧化碳，这一反应可能涉及脱氢酶系统。
Methanol-C14 was rapidly metabolized by carrot tissue slices, pea cotyledons, soybean cotyledons, castor bean endosperm, beet storage tissues, and mature beet leaves. With the exception of beet storage tissues, carbon dioxide was a chief product of methanol metabolism. In all tissues, methanol carbon was also incorporated in the organic acids, sugars, amino acids, and the insoluble residue. Serine, methionine, methionine sulfone, and methionine sulfoxide were important labelled components present in the amino acid fractions separated. Degradation of the serine-C14 that was produced by carrot tissues metabolizing methanol-C14 shown that the bulk of the label was present in the 3-position. The results are interpreted as indicating that methanol can act as a precursor of the carbon-1 units that are to be utilized in transmethylation reactions leading to serine and methionine biosynthesis. In addition, methanol can be oxidized to carbon dioxide by these tissues, and this reaction possibly involves dehydrogenase systems.