Aspergillus carbonarius as the main source of ochratoxin A contamination in dried vine fruits from the Spanish market
Aspergillus carbonarius as the main source of ochratoxin A contamination in dried vine fruits from the Spanish market
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DOI：
10.4315/0362-028x-66.3.504
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发表时间：
2003-03-01
影响因子：
2
通讯作者：
Cabañes, FJ
中科院分区：
文献类型：
--
作者：
Abarca, ML;Accensi, F;Cabañes, FJ
赭曲霉毒素A（OTA）可以出现在各种食物中，但在各种来源的葡萄干中检测到的浓度出乎意料地高。欧盟最近为这些食品规定了10微克/公斤的最大OTA限制。为了确定OTA的可能来源，对代表西班牙市场的50个干果样品（葡萄干、葡萄干和小葡萄干）进行了真菌学研究。50份样本中有49份（98%）检测到真菌污染。所有阳性标本均分离到黑色曲霉菌。尼日尔曲霉泡从98%的样品中分离出黑尼日尔，并且在58%的样品中发现了炭曲霉。168 A.尼日尔湾尼日尔菌株和91株A.对carbonarius分离株筛选其产生OTA的能力。88株（96.7%）A. carbonarius分离株和A.尼日尔湾发现尼日尔分离物是OTA产生者。黑曲霉为优势真菌。在黑曲霉中，A. carbonarius已经显示出产生OTA的一致能力，并且是这些底物中该真菌毒素的最可能来源。
Ochratoxin A (OTA) can occur in a wide range of foods, but unexpectedly high concentrations have been detected in dried vine fruits of various origins. The European Union has recently established a maximum OTA limit of 10 mug/kg for these foodstuffs. In order to determine the likely origin of OTA, a mycological study of 50 dried fruit samples (currants, raisins, and sultanas) representative of the Spanish market was conducted. Fungal contamination was detected in 49 of 50 (98%) samples. Black aspergilli were isolated from all of the positive samples. Aspergillus niger var. niger was isolated from 98% of the samples, and Aspergillus carbonarius was found in 58% of the samples. One hundred sixty-eight A. niger var. niger isolates and 91 A. carbonarius isolates were screened for their ability to produce OTA. Eighty-eight (96.7%) A. carbonarius isolates and one (0.6%) A. niger var. niger isolate were found to be OTA producers. Black aspergilli were the dominant fungi. Among black aspergilli, A. carbonarius has shown a consistent ability to produce OTA and is the most probable source of this mycotoxin in these substrates.