[Study on protective effect of vitamin E for ovarian grandlose cells and its mechanism in aged rats].
[Study on protective effect of vitamin E for ovarian grandlose cells and its mechanism in aged rats].
复制标题
维生素E对老年大鼠卵巢大细胞的保护作用及其机制研究
DOI：
--
复制
发表时间：
2009
影响因子：
--
通讯作者：
L. Yao
中科院分区：
文献类型：
--
作者：
Chang;Yan;Run;Ya;L. Yao
目的
观察维生素E（VE）对老年雌性大鼠卵巢凋亡相关蛋白Bcl-2、Bax的影响及其抗氧化能力的影响，研究VE对卵巢的延缓衰老作用及机制。
方法
自然衰老雌性大鼠给予不同剂量的外源VE。采用免疫组化方法和Western blot检测卵巢大损失细胞中凋亡调节蛋白Bcl-2、Bax的表达。采用生化方法检测血清总超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量。
结果
与成人对照组相比，老年对照组Bcl-2表达水平较低，Bax表达水平较高（P < 0.01），血清SOD活性降低，MDA水平显着升高（P < 0.01）。与老年对照组比较，VE组Bcl-2表达水平升高，Bax表达水平降低（P < 0.05），MDA表达水平显着降低（P < 0.01）。
结论
VE可调节凋亡相关蛋白Bcl-2、Bax的表达，对抗自由基损伤，对卵巢肥大细胞具有保护作用。
AIM
To observe the effect of vitamin E (VE) on ovarian apoptosis-related protein Bcl-2 and Bax and its impact on antioxidant capacity in aged female rats and to study the senility-delaying effect and mechanism of VE on ovary.
METHODS
Natural aging female rats were given different doses of exogenous VE. Then apoptosis regulatory protein Bcl-2, Bax expression in ovarian grandlose cells were detected by using immunohistochemical methods and Western blot. The contents of serum total superoxide dismutase (SOD) activity and malondialdehyde (MDA) were detected by using biochemical methods.
RESULTS
Contrasted with adult control group, the level of Bcl-2 expression in Senile control group was lower and the level of Bax expression was higher (P < 0.01), Serum SOD activity decreased and the level of MDA significantly increased (P < 0.01). Contrasted with senile control group, the level of Bcl-2 expression increased in VE group, the level of Bax expression decreased (P < 0.05), the level of MDA expression significantly decreased (P < 0.01).
CONCLUSION
VE can regulate apoptosis-related protein Bcl-2, Bax expression and confront free radical damage which contribute to a protective effect for ovarian grandiose cells.