Second harmonic generation microscopy: principles and applications to disease diagnosis
Second harmonic generation microscopy: principles and applications to disease diagnosis
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DOI：
10.1002/lpor.200910024
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发表时间：
2011-01-01
影响因子：
11
通讯作者：
Dong, Chen-Yuan
中科院分区：
文献类型：
--
作者：
Campagnola, Paul J.;Dong, Chen-Yuan
在这篇综述中，作者讨论了二次谐波产生的基本原理和影响发电特性的具体因素，并描述了二次谐波产生仪器的基本组成部分。此外，还介绍了倍频显微镜在生物学和医学不同领域的最新进展和影响。作者特别关注与连接组织、肌肉骨骼疾病和上皮癌相关的疾病诊断和基础研究。演讲最后对 SHG 显微镜的未来技术发展和需要解决的其他前沿问题提出了展望。[图形]20（左）、40（中）和 70（右）岁患者离体人类真皮的 SHG 和多光子自发荧光成像，其中蓝色 = SHG，绿色 = 自发荧光。
In this review, the authors discuss the underlying principles of SHG and specific factors that affect the generation properties and describe the essential components of a SHG instrument. In addition, results on the recent progress and impact SHG microscopy has made in different areas of biology and medicine are presented. In particular, the authors focus on disease diagnosis and basic research associated with connected tissues, musculo-skeletal disorders, and epithelial cancers. The presentation is concluded by offering a perspective on the future technical development of SHG microscopy and additional forefronts to be addressed.[GRAPHICS]SHG and multiphoton autofluorescence imaging of ex-vivo human dermis in 20 (left), 40 (middle), and 70 (right) years-old patients, where blue = SHG and green = autofluorescence.