Oct4 and Sox2 are overexpressed in human neuroblastoma and inhibited by chemotherapy
Oct4 and Sox2 are overexpressed in human neuroblastoma and inhibited by chemotherapy
复制标题
Oct4 和 Sox2 在人神经母细胞瘤中过度表达并被化疗抑制
DOI：
10.3892/or.2012.1765
复制
发表时间：
2012-07-01
期刊：
影响因子：
4.2
通讯作者：
Xiao, Xianmin
中科院分区：
文献类型：
--
作者：
Yang, Shaobo;Zheng, Jicui;Xiao, Xianmin
神经母细胞瘤（NB）是儿童时期最常见的颅外实体瘤，也是婴儿期最常诊断的肿瘤。Oct4和Sox 2是胚胎发育的重要转录因子，在决定干细胞命运中起着关键作用。本研究旨在探讨Oct4和Sox 2在NB组织中的表达及其与各种临床病理参数的关系。采用实时荧光定量PCR检测65例NB及癌旁组织中Oct4和Sox 2的表达。评估Oct4和Sox 2表达与临床数据之间的关系。采用免疫印迹和免疫组化方法检测Oct4和Sox 2蛋白的表达。结果发现，Oct4和Sox 2在NB组织中的表达水平明显高于癌旁组织（P
Neuroblastoma (NB) is the most common extracranial solid tumor in childhood and the most frequently diagnosed neoplasm during infancy. Oct4 and Sox2 are essential transcription factors for embryonic development and play key roles in determining the fate of stem cells. In this study, we aimed to investigate the expression of Oct4 and Sox2 in NB tissues, and evaluated their relationship with various clinicopathological parameters. Oct4 and Sox2 expression in 65 samples of NB and paracancerous tissues was examined by real-time PCR. The relationship between Oct4 and Sox2 expression and clinical data was assessed. To detect Oct4 and Sox2 expression at the protein level, western blot analyses and immunohistochemical staining were employed. We found that the expression levels of Oct4 and Sox2 in NB tissues were significantly higher than those in paracancerous tissues (P