Altered gene expression in the adult brain of fyn-deficient mice
Altered gene expression in the adult brain of fyn-deficient mice
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DOI：
10.1023/b:cemn.0000012720.71630.14
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发表时间：
2004-02-01
影响因子：
4
通讯作者：
Yamamoto, T
中科院分区：
文献类型：
--
作者：
Goto, J;Tezuka, T;Yamamoto, T
1. Fyn 是 Src 家族酪氨酸激酶的成员，与大脑发育和成人大脑功能有关。最近的研究已经确定了一些 Fyn 底物，然而，人们对 Fyn 介导的信号通路的转录靶点知之甚少。在本研究中，我们试图确定体内Fyn下游的靶标。2．我们使用包含超过12,000个基因的基因芯片比较了野生型和fyn缺陷小鼠的成年海马中表达的基因。3．结果显示，这些小鼠之间有 559 个转录本存在差异表达。 fyn缺陷小鼠中包括大量髓磷脂相关基因在内的20个基因的表达受到强烈抑制。 4．实时PCR和RNA印迹也证实fyn缺陷小鼠中这些髓磷脂相关基因（例如MBP和MOG）的表达降低，认为Fyn对于少突胶质细胞的功能和发育很重要。5．对 fyn 缺陷小鼠中不同表达的基因的进一步分析可能有助于揭示 Fyn 调节成年神经功能的分子机制。
1. Fyn, a member of Src-family tyrosine kinases, is implicated in both brain development and adult brain function. Recent studies have identified some Fyn substrates, however, little is known about the transcriptional targets for Fyn mediated signaling pathways. In the present study, we sought to identify targets downstream of Fyn in vivo.2. We compared genes expressed in adult hippocampi of wild-type and fyn-deficient mice using gene chips containing more than 12,000 genes.3. The results showed that 559 transcripts were expressed differentially between these mice. Expression of 20 genes including a substantial number of myelin-associated genes was strongly repressed in fyn-deficient mice.4. Reduced expression of these myelin-associated genes, such as MBP and MOG, in fyn-deficient mice was also confirmed by real-time PCR and northern blotting, arguing that Fyn is important for function and development of oligodendrocytes.5. Further analysis of the genes that are differently expressed in fyn-deficient mice may shed light on the molecular mechanism by which Fyn regulates adult neural function.