Sialidases play a key role in infection and anaemia in Trypanosoma congolense animal trypanosomiasis
Sialidases play a key role in infection and anaemia in Trypanosoma congolense animal trypanosomiasis
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DOI：
10.1111/j.1462-5822.2011.01730.x
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发表时间：
2012-03-01
影响因子：
3.4
通讯作者：
Baltz, Theo
中科院分区：
文献类型：
--
作者：
Coustou, Virginie;Plazolles, Nicolas;Baltz, Theo
非洲动物锥虫病是牲畜生产力的主要制约因素，并对非洲发展中国家的数百万人产生重要影响。这种主要由刚果锥虫引起的寄生虫病导致严重贫血，导致动物死亡。为了确定抗疫疫苗的潜在靶点，我们研究了刚果热锥虫的一个多基因反式唾液酸酶家族（TcoTS）。首次定量测定了唾液酸酶和反式唾液酸酶活性，以及在整个生命周期中严格调控的TcoTS表达模式。活性酶在寄生虫的血液中表达，并在感染期间释放到血液中。利用遗传工具，我们证明了在刚果弓形虫实验感染期间，TcoTS沉默与毒力损伤之间存在显著相关性。TcoTS表达降低影响感染性、寄生虫血症和发病机制的发展。利用重组tcot进行的免疫激发实验突出了它们在抗疾病疫苗中的潜在保护作用。
Animal African trypanosomiasis is a major constraint to livestock productivity and has an important impact on millions of people in developing African countries. This parasitic disease, caused mainly by Trypanosoma congolense, results in severe anaemia leading to animal death. In order to characterize potential targets for an anti-disease vaccine, we investigated a multigenic trans-sialidase family (TcoTS) in T. congolense. Sialidase and trans-sialidase activities were quantified for the first time, as well as the tightly regulated TcoTS expression pattern throughout the life cycle. Active enzymes were expressed in bloodstream form parasites and released into the blood during infection. Using genetic tools, we demonstrated a significant correlation between TcoTS silencing and impairment of virulence during experimental infection with T. congolense. Reduced TcoTS expression affected infectivity, parasitaemia and pathogenesis development. Immunization-challenge experiments using recombinant TcoTS highlighted their potential protective use in an anti-disease vaccine.