Platelet-derived growth factor activates mitogen-activated protein kinase in isolated caveolae
Platelet-derived growth factor activates mitogen-activated protein kinase in isolated caveolae
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DOI：
10.1073/pnas.94.25.13666
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发表时间：
1997-12-09
影响因子：
11.1
通讯作者：
Anderson, RGW
中科院分区：
文献类型：
--
作者：
Liu, PS;Ying, YS;Anderson, RGW
由于细胞中信号转导分子的模块化组织，肽激素影响许多不同细胞内靶点的能力被认为是可能的。然而，后生动物细胞中存在信号转导模块的证据是不完整的。在此，我们表明，形态学和细胞分级分离，促分裂原活化蛋白激酶途径的所有成分都集中在小窝的未刺激的人成纤维细胞，血小板源性生长因子Po无论是完整的细胞或小窝从这些细胞分离刺激酪氨酸磷酸化和激活有丝分裂原活化蛋白激酶小窝。因此，激酶激活的分子机制是预先组织在静止细胞的细胞表面的。
The ability or a peptide hormone to affect many different intracellular targets is thought to be possible because of the modular organization of signal transducing molecules in the cell, Evidence for the presence of signaling modules in metazoan cells, however, is incomplete. Herein we show, with morphology and cell fractionation, that all the components of a mitogen-activated protein kinase pathway are concentrated in caveolae of unstimulated human fibroblasts, Addition of platelet-derived growth factor Po either the intact cell or caveolae isolated from these cells stimulates tyrosine phosphorylation and activates mitogen-activated protein kinases in caveolae. The molecular machinery for kinase activation, therefore, is preorganized al the cell surface of quiescent cells.