Intensity-modulated nanoplasmonic interferometric sensor for MMP-9 detection.
Intensity-modulated nanoplasmonic interferometric sensor for MMP-9 detection.
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DOI：
10.1039/c8lc01391h
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发表时间：
2019-03
期刊：
影响因子：
6.1
通讯作者：
Yifeng Qian;Xie Zeng;Yongkang Gao;Hang Li;Sushil Kumar;Qiaoqiang Gan;Xuanhong Cheng;F. Bartoli
中科院分区：
文献类型：
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作者：
Yifeng Qian;Xie Zeng;Yongkang Gao;Hang Li;Sushil Kumar;Qiaoqiang Gan;Xuanhong Cheng;F. Bartoli
为了阐明免疫细胞的分泌功能，我们开发了一种基于强度询问的纳米等离子体圆形干涉生物传感器，用于分子分泌的无标记和动态传感。通过耦合自由光和表面等离子体激元 (SPP) 波，展示了卓越的灵敏度，当白光照射时，它会产生具有高对比度和窄线宽的相长和解构干涉图案。或者，在这项工作中采用窄带 LED 光源和 CCD 相机，通过简单的共线光学装置同时监测多个传感单元的传输强度。该强度调制传感平台的分辨率为 4.1 × 10-5 折射率单元 (RIU)，具有 1 s 的高时间分辨率，单个传感单元的小型化占地面积小至 9.8 × 9.8 μm2。通过集成多个传感器单元的信号，发现12×12传感器阵列的分辨率达到7.3×10-6 RIU。我们应用该传感器阵列在脂多糖（LPS）模拟后的不同时间点检测人单核细胞THP-1的基质金属蛋白酶9（MMP-9）分泌，结果与酶联免疫吸附测定（ELISA）测试非常一致，但不需要标记。研究发现传感器阵列的空间、时间和质量分辨率超过了其他无标记技术。这些生物分子阵列集成在微流体传感器平台中，对于研究细胞分泌信号的动力学和相互作用以及更好地了解单细胞功能具有巨大的潜力。
To elucidate the secretary function of immune cells, we develop a nanoplasmonic circular interferometric biosensor based on intensity interrogation for label-free and dynamic sensing of molecular secretion. Exceptional sensitivity has been demonstrated through coupling free light and surface plasmon polariton (SPPs) waves, which generates a constructive and deconstructive interference pattern with high contrast and narrow linewidth when illuminated by white light. Alternatively, by adopting a narrow-band LED source and a CCD camera in this work, the transmission intensity of multiple sensing units is monitored simultaneously with a simple collinear optical setup. This intensity-modulated sensing platform yields a resolution of 4.1 × 10-5 refractive index unit (RIU) with a high temporal resolution of 1 s and a miniaturized footprint as small as 9.8 × 9.8 μm2 for a single sensing unit. By integrating the signals from multiple sensor units, the resolution of a 12 × 12 sensor array was found to reach 7.3 × 10-6 RIU. We apply this sensor array to detect matrix metalloproteinase 9 (MMP-9) secretion from human monocytic cells, THP-1, at different time points after lipopolysaccharide (LPS) simulation and the results are in good agreement with enzyme-linked immunosorbent assay (ELISA) tests, but without the need for labeling. The spatial, temporal and mass resolutions of the sensor array are found to exceed other label-free technologies. These biomolecular arrays, incorporated in a microfluidic sensor platform, hold great potential for the study the dynamics and interplay of cell secretion signals and achieving a better understanding of single cell functions.