Purification of transforming growth factor beta 1 from human platelets.
Purification of transforming growth factor beta 1 from human platelets.
复制标题
从人血小板中纯化转化生长因子β1。
作者：
O. Werz;M. Brungs;D. Steinhilber
转化生长因子-β（TGF β）是一种生长和分化因子，可以从许多细胞类型中释放。在以前的研究中，血小板被确定为TGF β的丰富来源。本文介绍了一种从人血小板中提取转化生长因子β（TGF β）的快速、简便的方法，包括酸-乙醇提取、凝胶过滤、阳离子交换和反相层析。所有纯化步骤均在酸性条件下进行，以防止TGF β吸附到小瓶壁上。此外，使用挥发性溶剂和缓冲液，其允许通过冻干容易地去除溶剂和盐。使用这种方法，可以容易地从20单位的血小板浓缩物中以高产率（370微克）获得纯TGF β。
Transforming growth factor-beta (TGF beta) is a growth and differentiation factor which can be released from many cell types. In previous studies, platelets were identified as a rich source of TGF beta. Here we present a rapid and convenient method for TGF beta purification from human platelets which includes acid-ethanol extraction and gelfiltration, cation exchange and reversed phase chromatography. All purification steps are performed under acidic conditions to prevent adsorption of TGF beta to the vial walls. In addition, volatile solvents and buffers were used which allowed easy removal of solvent and salt by lyophilization. Using this method pure TGF beta can be easily obtained in high yield (370 micrograms) from 20 units of platelet concentrate.