Immunization of dissociated spleen cell cultures from normal mice.
Immunization of dissociated spleen cell cultures from normal mice.
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DOI：
10.1084/jem.126.3.423
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发表时间：
1967-09-01
期刊：
影响因子：
--
通讯作者：
Dutton RW
中科院分区：
文献类型：
--
作者：
Mishell RI;Dutton RW
本文描述了一种小鼠脾细胞悬液的培养系统。该系统为初次接触异源红细胞的体外免疫提供了充分的条件。在体外反应密切平行，在体内观察到的大小，早期动力学，抗原剂量，和被动抗体的抑制作用。培养细胞的反应在两个方面与体内观察到的不同。有一个增加的能力，以区分不同品种的同源红细胞和体外反应似乎不受任何机制调节体内反应的限制。
A culture system for cell suspensions from mouse spleens has been described. The system provides adequate conditions for in vitro immunization on initial exposure to heterologous erythrocytes. The in vitro response closely parallels that observed in vivo with respect to size, early kinetics, antigen dose, and the inhibitory effect of passive antibody. The response of cultured cells differs in two respects from that seen in vivo. There is an increase in the ability to discriminate between different varieties of homologous erythrocytes and the in vitro response does not appear to be limited by whatever mechanisms regulate the in vivo response.