Altered expression pattern of heat shock transcription factor, Y chromosome (HSFY) may be related to altered differentiation of spermatogenic cells in testes with deteriorated spermatogenesis
Altered expression pattern of heat shock transcription factor, Y chromosome (HSFY) may be related to altered differentiation of spermatogenic cells in testes with deteriorated spermatogenesis
复制标题
DOI：
10.1016/j.fertnstert.2006.01.053
复制
发表时间：
2006-09-01
影响因子：
6.7
通讯作者：
Iwamoto, Teruaki
中科院分区：
文献类型：
--
作者：
Sato, Yoko;Yoshida, Kaoru;Iwamoto, Teruaki
目的：评估热休克转录因子Y染色体（HSFY）在精子发生恶化的睾丸中的表达模式。设计：前瞻性研究。设置：大学医院，其分支医院和学术实验室。患者：因不孕症接受睾丸活检的男性和因睾丸癌接受睾丸切除术的男性。干预措施：经病理学评估后，将标本分为三组：正常精子发生（n = 8），成熟停滞（n = 5）和Sertoli细胞综合征（n = 4）。免疫组化和Western blotting技术测定了HSFY的表达。主要结果测量：睾丸中HSFY的表达。结果：Western blotting数据显示，正常精子发生、成熟停滞和支持细胞综合征的睾丸组织中存在HSFY，但成熟停滞和支持细胞综合征样本中的蛋白量发生了改变。免疫组化结果显示，HSFY在所有标本的生精细胞和支持细胞中均有表达。HSFY在成熟停滞组和Sertoli细胞综合征组中的表达率不同，而在成熟停滞组中的表达率较低或缺失。结论：HSFY在睾丸生精功能减退的组织中的表达改变可能与生精细胞分化的改变有关。
Objective: To evaluate the expression patterns of heat shock transcription factor, Y chromosome (HSFY), in the testes showing deteriorated spermatogenesis.Design: Prospective study.Setting: University hospital, its branch hospital, and academic laboratory.Patient(s): Men undergoing testicular biopsy for the investigation of infertility and men undergoing orchiectomy for testicular cancer.Intervention(s): After pathologic evaluation, specimens were subdivided into three groups: normal spermatogenesis (n = 8), maturation arrest (n = 5), and Sertoli cell-only syndrome (n = 4). Immunostaining and Western blotting techniques determined the expression of HSFY.Main Outcome Measure(s): Expression of HSFY in testes.Result(s): Western blotting data revealed HSFY in the testicular tissues with normal spermatogenesis, maturation arrest, and Sertoli cell-only syndrome, but the amount of the protein in the maturation arrest and Sertoli-cell only syndrome samples was altered. The immunohistochemical data demonstrated that HSFY was expressed in spermatogenic cells and Sertoli cells in all specimens. However, the expression of HSFY was low or absent in spermatogenic cells of maturation arrest specimens, and the ratio of HSFY expressed in Sertoli cells was different in the specimens with maturation arrest and with Sertoli cell-only syndrome.Conclusion(s): Altered expression of the HSFY in the testis showing deteriorated spermatogenesis may be associated with alteration of spermatogenic cell differentiation.