Alkaline phosphatase immobilization onto Bio-Gide® and Bio-Oss® for periodontal and bone regeneration
Alkaline phosphatase immobilization onto Bio-Gide® and Bio-Oss® for periodontal and bone regeneration
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DOI：
10.1111/j.1600-051x.2012.01877.x
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发表时间：
2012-06-01
影响因子：
6.7
通讯作者：
Jansen, John A.
中科院分区：
文献类型：
--
作者：
Oortgiesen, Daniel A. W.;Plachokova, Adelina S.;Jansen, John A.
目的评价碱性磷酸酶（ALP）在体外固定化Bio-Gide (R)上的效果，研究富集ALP的Bio-Gide (R)和/或Bio-Oss (R)在体内促进牙周再生的性能。材料与方法将碱性磷酸酶ALP固定在Bio-Gide (R)和Bio-Oss (R)上。48只大鼠接受牙周缺损治疗，分别采用Bio-Gide (R)、Bio-Gide (R)-ALP、Bio-Gide (R)-ALP/Bio-Oss (R)、Bio-Gide (R)/Bio-Oss (R)-ALP、Bio-Gide (R)-ALP/Bio-Oss (R)-ALP或空白治疗。进行显微ct和组织学分析。结果通过矿化能力评估，确定了与alp沉积时间相近的30 min为最佳时间，并将其作为Bio-Gide (R)-ALP膜进行动物实验。体内实验结果显示，类似2周后，缺损和植入材料仍然可见，存在炎症反应，膜降解正在进行。虽然有限，但在大多数Bio-Gide (R)-ALP标本和所有Bio-Gide (R)/Bio-Oss (R)-ALP标本中观察到骨形成，与Bio-Gide (R)和空白对照相比，骨形成明显更高。6周后，缺陷和颗粒仍然可见，而膜完全降解。Bio-Gide (R)-ALP治疗的缺损炎症反应降低，骨形成明显优于对照组。结论ALP固定在引导组织再生(GTR)/引导骨再生（GBR）材料(Bio-Gide （R)和Bio-Oss (R)）上，可以提高这些材料在GTR/GBR手术中的性能。
Aim To evaluate the effect of alkaline phosphatase (ALP) immobilization onto Bio-Gide (R) in vitro, and to study the in vivo performance of ALP-enriched Bio-Gide (R) and/or Bio-Oss (R) with the purpose to enhance periodontal regeneration. Materials and Methods Alkaline phosphatase ALP was immobilized onto Bio-Gide (R) and Bio-Oss (R). Forty-eight rats received periodontal defects, which were treated according to one of the following strategies: Bio-Gide (R), Bio-Gide (R)-ALP, Bio-Gide (R)-ALP/Bio-Oss (R), Bio-Gide (R)/Bio-Oss (R)-ALP, Bio-Gide (R)-ALP/Bio-Oss (R)-ALP, or empty. Micro-CT and histological analysis were performed. Results A 30 similar to min ALP-deposition time was determined as optimal from mineralization capacity assessment and consequently used as Bio-Gide (R)-ALP membranes in the animal experiment. In vivo results showed that after 2 similar to weeks, the defect and implanted materials were still visible, an inflammatory response was present, and membrane degradation was ongoing. Bone formation, although limited, was observed in the majority of Bio-Gide (R)-ALP specimens and all of the Bio-Gide (R)/Bio-Oss (R)-ALP specimens, and was significantly higher compared with Bio-Gide (R) and empty controls. After 6 similar to weeks, the defects and particles were still visible, whereas membranes were completely degraded. The inflammatory response was decreased and bone formation appeared superior for Bio-Gide (R)-ALP treated defects. Conclusion Immobilization of ALP onto guided tissue regeneration (GTR)/ guided bone regeneration (GBR)-materials (Bio-Gide (R) and Bio-Oss (R)) can enhance the performance of these materials in GTR/GBR procedures.