SELECTIVE DENDRITIC TRANSPORT OF RNA IN HIPPOCAMPAL-NEURONS IN CULTURE
SELECTIVE DENDRITIC TRANSPORT OF RNA IN HIPPOCAMPAL-NEURONS IN CULTURE
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DOI：
10.1038/330477a0
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发表时间：
1987-12-03
期刊：
影响因子：
64.8
通讯作者：
STEWARD, O
中科院分区：
文献类型：
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作者：
DAVIS, L;BANKER, GA;STEWARD, O
典型的中枢神经系统（CNS）的神经元在树突上的突触接触部位精心制作了成千上万的膜特化。为了构建、维持和修改这些特化，神经元必须产生适当的分子成分并将其传递到特定的突触位点。先前的研究表明，多核糖体选择性地位于突触后位点下方1,2，这表明在神经元中，与其他细胞类型一样3 - 8,19，蛋白质合成机制位于需要特定蛋白质的位点或附近。因此，将核糖体和信使RNA运送到细胞内特定目的地的机制引起了相当大的兴趣。在这里，我们描述了一个RNA在树突中的运输系统，该系统可以为核糖体和mRNA传递到树突中的突触位点提供一种机制。培养的海马神经元在细胞核中加入3h -尿苷，然后以大约0.5 mm / d的速度选择性地将新合成的RNA转运到树突中。这种转运受到代谢性毒物的抑制，这表明它是一个主动的、依赖能量的过程。RNA可能与细胞骨架一起运输。
Typical neurons of the central nervous system (CNS) elaborate tens of thousands of membrane specializations at sites of synaptic contacts on their dendrites. To construct, maintain, and modify these specializations, neurons must produce and deliver the appropriate molecular constituents to particular synaptic sites. Previous studies have revealed that polyribosomes are selectively positioned beneath postsynaptic sites1,2suggesting that in neurons, as in other cell types3–8,19, protein synthetic machinery is located at or near the sites where particular proteins are needed. The mechanisms that deliver ribosomes and messenger RNA to their specific destinations in cells are therefore of considerable interest. Here we describe a system for RNA transport in dendrites that could provide a mechanism for the delivery of ribosomes and mRNA to synaptic sites in dendrites. Hippocampal neurons grown in culture incorporate3H-uridine in the nucleus, then selectively transport the newly synthesized RNA into dendrites at a rate of about 0.5 mm day–1. The transport is inhibited by metabolic poisons, suggesting that it is an active, energy-dependent process. The RNA may be transported in association with the cytoskeleton.