Membranolysis by the ninth component of human complement.
Membranolysis by the ninth component of human complement.
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人体补体第九种成分的膜溶解作用。
DOI：
10.1016/0006-291x(81)91981-1
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发表时间：
1981
影响因子：
3.1
通讯作者：
Podack,ER
中科院分区：
文献类型：
--
作者：
Tschopp,J;Podack,ER
通过加热（48°C）诱导C9聚合，然后将C9暴露于含有羧基荧光素的卵磷脂囊泡中，证明了分离的C9的裂解性能。在这种环境下，C9穿透囊泡并释放标记物。C5b-9和C5b-8在48°C时也释放羧基荧光素，C5b-7的释放程度较轻。然而，分离的C5b-6、C7、C8和白蛋白均未引起这种释放。在30℃时，C9仍以单体形式存在，不能裂解囊泡。在48℃下形成的C9聚合物呈环状，内径约为100 Å，外径约为180 Å。从顶部看，这些C9聚合物的环与补体的膜攻击复合物形成的超微结构惊人地相似。我们的结果表明，聚合物C9引起磷脂囊泡的膜溶解，因此，C9单独是一种细胞毒性分子。
The lytic property of isolated C9 was shown by using heat (48°C) to induce polymerization of C9, which was then exposed to egg lecithin vesicles containing carboxyfluorescein. In this environment, C9 penetrated the vesicles and released the marker. C5b-9 and C5b-8 also released carboxyfluorescein at 48°C, as did C5b-7 to a lesser extent. However, neither isolated C5b-6, C7, C8 nor albumin caused such release. At 30°C, the C9 remained in the monomeric form and could not lyse the vesicles.C9 polymers formed at 48°C were ring-shaped with an internal diameter of approximately 100 Å and an outer diameter of approximately 180 Å. These rings of C9 polymers strikingly resembled the ultrastructures formed by the membrane attack complex of complement, viewed from the top.Our results indicate that polymeric C9 causes the membranolysis of phospholipid vesicles and, hence, that C9 alone is a cytotoxic molecule.