CHROMOSOME ASSEMBLY INVITRO - TOPOISOMERASE-II IS REQUIRED FOR CONDENSATION
CHROMOSOME ASSEMBLY INVITRO - TOPOISOMERASE-II IS REQUIRED FOR CONDENSATION
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DOI：
10.1016/0092-8674(91)90215-k
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发表时间：
1991-01-11
期刊：
影响因子：
64.5
通讯作者：
LAEMMLI, UK
中科院分区：
文献类型：
--
作者：
ADACHI, Y;LUKE, M;LAEMMLI, UK
通过特异性免疫耗竭的方法，研究了非洲爪哇卵有丝分裂提取物中拓扑异构酶II(Topo II)在染色体凝聚中的作用。具有大量内源Topo II的HeLa核在Topo II耗尽的提取物中转化为有丝分裂染色体的情况与对照一样好。然而，鸡红细胞核中Topo II的含量非常低，在耗尽的提取物中不会转化为浓缩的染色体，尽管在加入纯化的Topo II后，它们的凝聚是正常的。剂量实验支持Topo II可能参与染色体凝聚的结构概念。在Topo II耗尽的抽提物中，红核进展为预缩缩染色体，缺乏核膜-板层复合体，由一簇肿胀的染色单体组成。
The role of topoisomerase II (topo II) in chromosome condensation was studied in a mitotic extract derived from Xenopus eggs by specific immunodepletion. HeLa nuclei, which have a high complement of endogenous topo II, are converted to mitotic chromosomes in the topo II-depleted extract equally well as in the control. Chicken erythrocyte nuclei, however, which have a very low content of topo II, do not convert to condensed chromosomes in the depleted extract, although their condensation is normal upon addition of purified topo II. Dosage experiments support the possible notion of a structural involvement of topo II in chromosome condensation. In the topo II-depleted extract the erythrocyte nuclei progress to precondensation chromosomes, which lack the nuclear membrane-lamina complex and consist of a cluster of swollen chromatids.