Upregulation of the Long Non-coding RNA PlncRNA-1 Promotes Esophageal Squamous Carcinoma Cell Proliferation and Correlates with Advanced Clinical Stage
Upregulation of the Long Non-coding RNA PlncRNA-1 Promotes Esophageal Squamous Carcinoma Cell Proliferation and Correlates with Advanced Clinical Stage
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长非编码RNA PlncRNA-1的上调促进食管鳞癌细胞增殖并与晚期临床分期相关
DOI：
10.1007/s10620-013-2956-7
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发表时间：
2014-03-01
影响因子：
3.1
通讯作者：
Cao, Xiu-Feng
中科院分区：
文献类型：
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作者：
Wang, Chun-Mei;Wu, Qing-Quan;Cao, Xiu-Feng
近年来的研究表明，长链非编码rna （lncRNAs）在癌症生物学中发挥着重要的调控作用。PlncRNA-1是与前列腺癌细胞凋亡和增殖相关的lncrna之一。目的探讨PlncRNA-1在食管鳞状细胞癌（ESCC）发病机制中的潜在作用。材料与方法采用定量实时聚合酶链反应（quantitative real-time polymerase chain reaction, qRT-PCR）检测PlncRNA-1在73对ESCC及其配对正常组织中的表达水平。分析PlncRNA-1与临床病理特征及临床分期的相关性。MTT、集落形成实验和流式细胞术检测PlncRNA-1敲除后癌细胞的增殖和凋亡情况。结果PlncRNA-1在人ESCC组织中的表达明显高于癌旁非癌组织（69.8%,p< 0.05），且PlncRNA-1的高表达与ESCC的临床分期（p< 0.01）和淋巴结转移（p< 0.05）有显著相关性。此外，在体外实验中，PlncRNA-1的敲低降低了细胞增殖，增加了细胞凋亡。结论splncrna -1在ESCC细胞增殖中起重要作用。PlncRNA-1过表达与肿瘤分期及淋巴结转移相关，可能是ESCC的潜在预后指标和治疗靶点。
BackgroundRecent studies revealed that long noncoding RNAs (lncRNAs) play critical regulatory roles in cancer biology. PlncRNA-1 is one of lncRNAs that is associated with cell apoptosis and proliferation of prostate cancer.AimThis study aimed to assess the potential role of PlncRNA-1 in the pathogenesis of esophageal squamous cell carcinoma (ESCC).Materials and MethodsQuantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression level of PlncRNA-1 in 73 pairs of ESCC and their matched normal tissues. The correlation of PlncRNA-1 with clinicopathological features and clinical stages was also analyzed. Cancer cell proliferation and apoptosis were assessed following knock-down of PlncRNA-1 by MTT, colony formation assay, and flow cytometry.ResultsThe expression of PlncRNA-1 was significantly higher in human ESCC compared with the adjacent noncancerous tissues (69.8 %,p< 0.05), and the high level of PlncRNA-1 expression was significantly correlated with advanced clinical stage (p< 0.01) and lymph node metastasis (p< 0.05). Furthermore, knockdown of PlncRNA-1 reduced cell proliferation and increased the apoptosis in vitro.ConclusionsPlncRNA-1 plays an important role in ESCC cell proliferation. Overexpression of PlncRNA-1 is correlated with advanced tumor stage and lymph node metastasis, and may serve as a potential prognostic marker and therapeutic target for ESCC.