New data on epizootiology and genetics of piroplasms based on sequences of small ribosomal subunit and cytochrome b genes
New data on epizootiology and genetics of piroplasms based on sequences of small ribosomal subunit and cytochrome b genes
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DOI：
10.1016/j.vetpar.2006.07.004
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发表时间：
2006-12-20
影响因子：
2.6
通讯作者：
Irwin, P. J.
中科院分区：
文献类型：
--
作者：
Criado, A.;MartineZ, J.;Irwin, P. J.
作为我们对西班牙和其他国家螺形体病分子流行病学研究的延续，我们在这篇论文中介绍了一些螺形体的新宿主的发现，以及它们的18S rRNA基因序列的信息。遗传数据补充了细胞色素b基因序列（只要可能）。从这些分子研究中得出以下结论：环状杆菌能够感染狗，因为它是在有症状的动物中被诊断出来的。根据细胞色素b序列，牛和狗的分离株存在轻微差异。然而，相同的分离株显示出相同的18S rRNA基因序列。这很好地说明了线粒体基因对检查亚特异性变异的有用性。牛巴贝斯虫是马的一种偶发寄生虫，因为在两匹有症状的马身上发现了这种寄生虫。我们发现了西班牙T马样和B. ovis分离株的18S rRNA基因存在多态性的证据。来自西班牙海鸥的bennetti与B. ovis有松散的关系，可能代表了一个基因上独特的巴贝斯德分支。首次在南非犬巴贝斯虫中获得细胞色素b假基因的部分序列。该假基因与双歧杆菌细胞色素b基因有远亲关系。这些新发现证实了一些梨状质体感染多个宿主的能力，以及细胞色素b基因存在相对广泛的遗传多态性。另一方面，梨质体中可能存在核位置的mtdna样假基因的存在是有趣的，因为它们可能对分子系统发育研究产生影响。(c) 2006 Elsevier B.V.版权所有
As a continuation of our studies on molecular epizootiology of piroplasmosis in Spain and other countries, we present in this contribution the finding of new hosts for some piroplasms, as well as information on their 18S rRNA gene sequences. Genetic data were complemented with sequences of apocytochrome b gene (whenever possible). The following conclusions were drawn from these molecular studies:Theileria annulata is capable of infecting dogs, since it was diagnosed in a symptomatic animal. According to cytochrome b sequences, isolates from cows and dog present slight differences. The same isolates showed, however, identical sequence in the 18S rRNA gene. This exemplifies well the usefulness of the mitochondrial gene for examining infra-specific variation.Babesia bovis is an occasional parasite of equines, since it was detected in two symptomatic horses.We found evidence of genetic polymorphism occurring in the 18S rRNA gene of Spanish T equi-like and B. ovis isolates.B. bennetti from Spanish seagull is loosely related to B. ovis, and might represent a genetically distinct branch of babesids.A partial sequence of a cytochrome b pseudogene was obtained for the first time in Babesia canis rossi from South Africa. The pseudogene is distantly related to B. bigemina cytochrome b gene.These new findings confirm the ability of some piroplasms to infect multiple hosts, as well as the existence of a relatively wide genetic polymorphisms with respect to the cytochrome b gene. On the other hand, the existence of mtDNA-like pseudogenes of possible nuclear location in piroplasms is interesting due to their possible impact on molecular phylogeny studies. (c) 2006 Elsevier B.V. All rights reserved.