Analysis using size exclusion chromatography of poly(N-isopropyl acrylamide) using methanol as an eluent.
Analysis using size exclusion chromatography of poly(N-isopropyl acrylamide) using methanol as an eluent.
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DOI：
10.1016/j.chroma.2017.05.050
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发表时间：
2017-07-28
期刊：
影响因子：
--
通讯作者：
Rimmer S
中科院分区：
文献类型：
--
作者：
Swift T;Hoskins R;Telford R;Plenderleith R;Pownall D;Rimmer S
用于聚合物分析的稳定的基于甲醇的SEC系统的首批实例之一。使用DOSY NMR测定通用校准以获得特性粘度。难交联支化聚丙烯酰胺的分析。尺寸排阻色谱法传统上在水性或非极性溶剂中进行。介绍了一种以甲醇为移动的相的聚合物摩尔质量分布的显示系统。这被证明是一个合适的系统，用于确定的摩尔质量分布聚（N-异丙基丙烯酰胺）（PNIPAM）;聚合物类，往往是难以分析的尺寸排阻色谱法。DOSY NMR用于提供特性粘度数据，其与粘度检测器结合使用以提供绝对校准。然后通过提供具有生物功能性端基的分散的高度支化的PNIPAM的绝对摩尔质量分布来显示该系统的实用性。
One of first examples of stable Methanol based SEC system for polymer analysis. Universal Calibration determined using DOSY NMR to get intrinsic viscosity. Analysis of difficult to characterise branched polyacrylamides. Size Exclusion Chromatography is traditionally carried out in either aqueous or non-polar solvents. A system to present molar mass distributions of polymers using methanol as a mobile phase is presented. This is shown to be a suitable system for determining the molar mass distributions poly(N-isopropylacrylamide)s (PNIPAM); a polymer class that is often difficult to analyze by size exclusion chromatography. DOSY NMR was used to provide intrinsic viscosity data that was used in conjunction with a viscometric detector to provide absolute calibration. Then the utility of the system was shown by providing the absolute molar mass distributions of dispersed highly branched PNIPAM with biologically functional end groups.