The establishment of follicular dominance in co-cultured mouse ovarian follicles
The establishment of follicular dominance in co-cultured mouse ovarian follicles
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DOI：
10.1530/jrf.0.1060001
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发表时间：
1996-01-01
期刊：
影响因子：
--
通讯作者：
Gosden, RG
中科院分区：
文献类型：
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作者：
Spears, N;deBruin, JP;Gosden, RG
为了更好地研究卵泡优势现象，建立了一个优势卵泡和从属卵泡的体外模型。小鼠腔前卵泡单独培养或成对培养。成对的卵泡在直接接触或共享介质中，但没有物理接触。实验表明，在允许卵泡之间接触的情况下，一个卵泡总是成为主导的，而另一个卵泡在培养期间几乎不会生长和发育。相反，在卵泡之间没有接触的情况下，共培养对卵泡的发育没有影响。因此，没有证据表明优势卵泡分泌的扩散因子可能会影响邻近卵泡的发育。在接触共培养6天后，组织学检查表明，附属卵泡是健康的，尽管在培养过程中几乎没有生长。在进一步的实验中，在共培养2天后(当大小已经有显著差异时)分离从属卵泡和主导卵泡，并单独培养。这些“释放的”卵泡在剩余的培养期间表现出激增的生长，在培养结束时达到与对照组几乎无法区分的大小和外观。这证实了在这个模型中，优势卵泡虽然抑制了其邻近卵泡的生长，但并没有导致从属卵泡发生不可逆转的闭锁。体外模型将使我们能够更详细地研究主从性卵泡相互作用的直接影响，并应该增加我们对这一鲜为人知的现象的了解。
An in vitro model of dominant and subordinate ovarian follicles was developed to allow a closer investigation of the phenomenon of follicular dominance. Preantral mouse ovarian follicles were cultured either alone or in pairs. Pairs of follicles were either in direct contact or in shared medium, but without physical contact. The experiments showed that where contact was allowed to develop between follicles one follicle invariably became dominant, while the other would grow and develop little during the culture period. In contrast, there was no effect of co-culture on follicle development in the absence of contact between the follicles. There was, therefore, no evidence of secretion of a diffusible factor by a dominant follicle that could affect the development of neighbouring follicles. After 6 days of co-culture with contact, histological examination of the subordinate follicle showed that it was healthy, in spite of remarkably little growth during culture. In a further experiment, the subordinate follicle was separated from the dominant one after 2 days of co-culture (when a significant difference in size had already developed), and cultured alone. These 'released' follicles exhibited a spurt of growth during the remaining culture period, attaining a size and appearance indistinguishable from those of controls by the end of culture. This confirms that the dominant follicle, while depressing the growth of its neighbour, is not inducing irreversible atresia in the subordinate follicle in this model. The in vitro model will allow a more detailed study of direct influences of dominant-subordinate follicle interactions, and should increase our knowledge of a poorly understood phenomenon.