Nucleosomes and neutrophil extracellular traps in septic and burn patients
Nucleosomes and neutrophil extracellular traps in septic and burn patients
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DOI：
10.1016/j.clim.2017.08.014
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发表时间：
2017-10-01
影响因子：
8.6
通讯作者：
Schattner, Mirta
中科院分区：
文献类型：
--
作者：
Kaufman, Tomas;Magosevich, Debora;Schattner, Mirta
NETosis是一种与炎症和组织损伤相关的宿主防御机制。实验模型显示血小板和血管性血友病因子（vonWillebrand factor，VWF）是血管内NETosis的关键因素。我们测定了脓毒症和烧伤患者入院后第1天和第4天（dpa）的NETosis。在败血症中，它们与人中性粒细胞弹性蛋白酶（HNE）-DNA复合物和SOFA评分在1 dpa相关，并与死亡率相关。患者的中性粒细胞有自发性NETosis，对刺激无反应。虽然两组血小板P-选择素和TNF-α均升高，但败血症患者在1dpa时血小板TLR-4表达、VWF水平和IL-6水平均较高。无论是血小板活化标志物还是细胞因子与核小体或HNE-DNA均无相关性，核小体可作为脓毒症患者器官损害的指标和死亡率的预测因子，但在烧伤患者中则无相关性。血小板活化、VWF和细胞因子似乎不是这些患者组中NETosis的关键介质。(C)2017由Elsevier Inc.出版
NETosis is a host defense mechanism associated with inflammation and tissue damage. Experimental models show that platelets and von Willebrand factor (VWF) are key elements for intravascular NETosis.We determined NETosis in septic and burn patients at 1 and 4 days post-admission (dpa).Nucleosomes were elevated in patients. In septics, they correlated with Human Neutrophil Elastase (HNE)-DNA complexes and SOFA score at 1 dpa, and were associated with mortality.Patient's neutrophils had spontaneous NETosis and were unresponsive to stimulation. Although platelet P-selectin and TNF-alpha were increased in both groups, higher platelet TLR-4 expression, VWF levels and IL-6 were found in septics at 1 dpa. Neither platelet activation markers nor cytokines correlated with nucleosomes or HNE-DNA.Nucleosomes could be indicators of organ damage and predictors of mortality in septic but not in burn patients. Platelet activation, VWF and cytokines do not appear to be key mediators of NETosis in these patient groups. (C) 2017 Published by Elsevier Inc.