EBOLA VIRUS - IDENTIFICATION OF VIRION STRUCTURAL PROTEINS
EBOLA VIRUS - IDENTIFICATION OF VIRION STRUCTURAL PROTEINS
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DOI：
10.1099/0022-1317-49-2-333
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发表时间：
1980-01-01
影响因子：
3.8
通讯作者：
JOHNSON, KM
中科院分区：
文献类型：
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作者：
KILEY, MP;REGNERY, RL;JOHNSON, KM
纯化的埃博拉病毒的聚丙烯酰胺凝胶电泳显示存在4种主要的病毒体结构蛋白，命名为VP 1、VP 2、VP 3和VP 4。水泡性口炎病毒（VSV）蛋白质用作MW标记物，并且病毒体蛋白质具有125，000（VP 1）、104，000（VP 2）、40，000（VP 3）和26，000（VP 4）的MW。VP 1被葡萄糖胺标记，可能是一种糖蛋白。在酒石酸钾中，埃博拉病毒粒子的密度约为1.14 μ g/ml。当用去污剂处理病毒体时，释放出在CsCl中密度为1.32 μ g/ml的病毒核衣壳。蛋白质VP 2和VP 3始终与释放的核衣壳相关，并且可能是与VSV的N蛋白类似的主要结构核衣壳蛋白。蛋白质VP 4在释放的核衣壳中减少或缺失，并且可能类似于VSV和类似病毒的膜（M）蛋白。糖蛋白（VP 1）比任何已知的负链RNA病毒的糖蛋白都大，并且不能很好地用35 S-甲硫氨酸标记。VP 1可被去污剂溶解，它可能是病毒粒子刺突的一个组分，类似于VSV的G蛋白。这些结果，结合埃博拉病毒粒子RNA的分析，强烈表明，该病毒是一种负链RNA病毒，沿着马尔堡病毒，可能构成一个新的分类群内。
Polyacrylamide gel electrophoresis of purified Ebola virus revealed the presence of 4 major virion structural proteins designated VP1, VP2, VP3 and VP4. Vesicular stomatitis virus (VSV) proteins were used as MW markers and the virion proteins had MW of 125,000 (VP1), 104,000 (VP2), 40,000 (VP3) and 26,000 (VP4). VP1 was labeled with glucosamine and is probably a glycoprotein. The density of the Ebola virion was about 1.14 g/ml in potassium tartrate. Virus nucleocapsids with a density of 1.32 g/ml in CsCl were released when virions were treated with detergents. Proteins VP2 and VP3 were consistently associated with released nucleocapsids and are probably the major structural nucleocapsid proteins analogous to the N protein of VSV. Protein VP4 was reduced or absent in released nucleocapsids and is probably analogous to the membrane (M) protein of VSV and similar viruses. The glycoprotein (VP1) is larger than the glycoprotein of any known negative-strand RNA virus and is not labeled well with 35S-methionine. VP1 is solubilized by detergent treatment, it may be a component of the virion spikes and analogous to the G protein of VSV. These results, in conjunction with analysis of Ebola virion RNA, strongly suggest that the virus is a negative-strand RNA virus and, along with Marburg virus, may constitute a new taxon within this group.