ENHANCED MEMBRANE-BINDING OF AUTOANTIBODIES TO CULTURED KERATINOCYTES OF SYSTEMIC LUPUS-ERYTHEMATOSUS PATIENTS AFTER ULTRAVIOLET-B ULTRAVIOLET-A IRRADIATION
ENHANCED MEMBRANE-BINDING OF AUTOANTIBODIES TO CULTURED KERATINOCYTES OF SYSTEMIC LUPUS-ERYTHEMATOSUS PATIENTS AFTER ULTRAVIOLET-B ULTRAVIOLET-A IRRADIATION
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DOI：
10.1172/jci115922
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发表时间：
1992-09-01
影响因子：
15.9
通讯作者：
KRUEGER, JG
中科院分区：
文献类型：
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作者：
GOLAN, TD;ELKON, KB;KRUEGER, JG
虽然已知阳光可引起系统性红斑狼疮(SLE)患者的皮肤损害，并加重全身症状，但其潜在机制仍不清楚。我们报道了经紫外线B(UVB)照射(200-1600J/m2)培养的SLE角质形成细胞中，有10个细胞株的免疫球蛋白自身抗体与细胞表膜结合增强的实验。与可溶性细胞内抗原Sm、RNP、SSA/Ro、SSb/La结合增强的自身抗体探针，而具有抗dsDNA活性的血清不显示这种结合。来自几个来源的对照角质形成细胞在紫外线照射后共享低水平的自身抗体结合。此外，4/6株对UVB敏感的SLE株在UVA照射(50-150kJ/m2)后，与SLE角质形成细胞表面的自身抗体结合增加，但幅度较小。当UVB敏感的非照射SLE株暴露于自体血清时，3/8的血清显示出显著的增加的免疫球蛋白结合率，并且在UVB暴露后进一步增加。患者角质形成细胞表面盐溶抗原表达增强，但非对照角质形成细胞可能部分解释了SLE患者的光敏性。
Although sunlight is known to induce skin lesions in patients with systemic lupus erythematosus (SLE) and to exacerbate systemic manifestations, the underlying mechanisms remain obscure. We report experiments that show enhanced binding of IgG autoantibodies to the cell surface membrane of ultraviolet-B(UVB) irradiated (200-1,600 J/m2) cultured SLE keratinocytes in 10 out of 12 such cell strains. The autoantibody probes showing increased binding were directed against the soluble intracellular antigens, Sm, RNP, SSA/Ro, SSB/La, whereas serum with anti-dsDNA activity did not demonstrate such binding. Control keratinocytes from several sources shared low level binding of autoantibodies after ultraviolet light exposure. In addition, 4/6 UVB-sensitive SLE strains showed increased autoantibody binding to the surface of SLE keratinocytes after UVA exposure (50-150 kJ/m2), but of lower magnitude. When UVB-sensitive nonirradiated SLE strains were exposed to autologous serum, 3/8 sera demonstrated a striking increase in IgG binding, which increased further after UVB exposure. Enhanced expression of saline-soluble intracellular antigens on the cell surface membrane of patient, but not control, keratinocytes may, in part, explain the photosensitivity of patients with SLE.