An RNA polymerase I termination site can stimulate the adjacent ribosomal gene promoter by two distinct mechanisms in Xenopus laevis.
An RNA polymerase I termination site can stimulate the adjacent ribosomal gene promoter by two distinct mechanisms in Xenopus laevis.
复制标题
在非洲爪蟾中，RNA 聚合酶 I 终止位点可以通过两种不同的机制刺激相邻的核糖体基因启动子。
DOI：
10.1101/gad.4.7.1240
复制
发表时间：
1990
影响因子：
10.5
通讯作者：
Reeder,RH
中科院分区：
文献类型：
--
作者：
McStay,B;Reeder,RH
在非洲爪蟾（Xenopus laevis）的核糖体基因中，T3终止子位于基因启动子5'边界上游约60bp处。我们之前已经证明，终止子的突变同时消除了终止子并损害了RNA聚合酶i的起始。在这里，我们表明终止子通过两种不同的机制影响启动子。在一种机制中，终止子通过阻止聚合酶读取起始复合物来保护启动子。在第二种机制中，终止子直接与启动子相互作用，无论终止是否发生。这种积极的相互作用需要终止子相对于启动子的精确定位，并且对终止子的移动敏感，只要1或2bp。我们得出结论，终止子和启动子作为一个相互依赖的复合体相互作用。
On the ribosomal genes of Xenopus laevis, the T3 terminator is located approximately 60 bp upstream of the 5' boundary of the gene promoter. We have shown previously that mutation of the terminator simultaneously abolishes termination and impairs initiation by RNA polymerase I. Here, we show that the terminator influences the promoter by two distinct mechanisms. In one mechanism the terminator protects the promoter by preventing polymerase from reading through the initiation complex. In a second mechanism, the terminator interacts directly with the promoter, whether or not termination occurs. This positive interaction requires precise positioning of the terminator relative to the promoter and is sensitive to movement of the terminator by as little as 1 or 2 bp. We conclude that the terminator and promoter interact as one interdependent complex.