Regulation of Streptococcus mutans PTS Bio by the transcriptional repressor NigR.
Regulation of Streptococcus mutans PTS Bio by the transcriptional repressor NigR.
复制标题
转录抑制因子 NigR 对变形链球菌 PTS Bio 的调节。
DOI：
10.1111/omi.12093
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发表时间：
2015
影响因子：
3.7
通讯作者：
Ajdic,D
中科院分区：
文献类型：
--
作者：
Vujanac,M;Iyer,VS;Sengupta,M;Ajdic,D
变形链球菌与人类龋病有关，其碳水化合物代谢在龋病的形成中起重要作用。糖的转运和代谢是变形链球菌在口腔中生存的关键。已知独特的磷酸烯醇丙酮酸-糖磷酸转移酶系统PTSBioof突变链球菌UA 159在蔗糖生长的生物膜中表达（Mol Oral Microbiol 28：2013; 114）。在这项研究中，我们分析了编码PTSBio的操纵子（OBio）的转录调控，并表明它被NigR（一种LacI样转录调控因子）抑制。使用电迁移率变动分析，我们描述了NigR以不同亲和力结合的两种操作子。我们还确定了转录起始位点，并表明其中一个运营商与启动子重叠，并推测抑制转录起始。突变分析揭示了操纵子中NigR高亲和力结合所需的关键核苷酸。PTSBio在变异链球菌生物膜中表达，因此了解其调控可能为龋病的治疗和预防提供改进的策略。
Streptococcus mutansis implicated in human dental caries, and the carbohydrate metabolism of this organism plays an important role in the formation of this disease. Carbohydrate transport and metabolism are essential for the survival ofS. mutansin the oral cavity. It is known that a unique phosphoenolpyruvate‐sugar phosphotransferase system PTSBioofS. mutansUA159 is expressed in sucrose‐grown biofilms (Mol Oral Microbiol28: 2013; 114). In this study we analyzed the transcriptional regulation of the operon (OBio) encoding the PTSBioand showed that it was repressed by NigR, a LacI‐like transcriptional regulator. Using electro‐mobility shift assay, we described two operators to which NigR bound with different affinities. We also identified the transcriptional start site and showed that one of the operators overlaps with the promoter and presumably represses initiation of transcription. Mutational analyses revealed the key nucleotides in the operators required for high‐affinity binding of NigR. PTSBiois expressed inS. mutansbiofilms so understanding its regulation may provide improved strategies for caries treatment and prevention.