Persistence of SARS-CoV-2 in Water and Wastewater
Persistence of SARS-CoV-2 in Water and Wastewater
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DOI：
10.1021/acs.estlett.0c00730
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发表时间：
2020-12-08
影响因子：
10.9
通讯作者：
Bibby, Kyle
中科院分区：
文献类型：
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作者：
Bivins, Aaron;Greaves, Justin;Bibby, Kyle
在感染个体的粪便中经常检测到严重的急性呼吸综合征2（SARS-COV-2）RNA。尽管以前在废水中尚未发现感染性SARS-COV-2，但已从至少一名患者的粪便中分离出感染性SARS-COV-2，这引起了人们对废水中感染性SARS-COV-2存在的担忧。水和废水中SARS-COV-2的命运和灭活特征尚不清楚，基于替代模型的当前失活估计值。在这项研究中，在水和废水中确定了SARS-COV-2感染性和RNA信号的持久性。在废水中降低90％的SARS-COV-2和室温下自来水的时代分别为1.5和1.7天。在高启动的滴度10（5）TCID50毫升（-1））实验中，感染性病毒持续了整个7天抽样时间过程。在50和70度C的废水中，感染性SARS-COV-2的观察到的T-90值分别降低至15分钟和2分钟。发现SARS-COV-2 RNA比感染性SARS-COV-2更持久，这表明仅RNA的环境检测并不能证实感染的风险。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA is frequently detected in the feces of infected individuals. While infectious SARS-CoV-2 has not previously been identified in wastewater, infectious SARS-CoV-2 has been isolated from the feces of at least one patient, raising concerns about the presence of infectious SARS-CoV-2 in wastewater. The fate and inactivation characteristics of SARS-CoV-2 in water and wastewater are unknown, with current inactivation estimates based on surrogate models. In this study, the persistence of SARS-CoV-2 infectivity and RNA signal was determined in water and wastewater. The times for 90% reduction (T-90) of viable SARS-CoV-2 in wastewater and tap water at room temperature were 1.5 and 1.7 days, respectively. In high-starting titer 10(5) TCID50 mL(-1)) experiments, infectious virus persisted for the entire 7-day sampling time course. In wastewater at 50 and 70 degrees C, the observed T-90 values for infectious SARS-CoV-2 were decreased to 15 and 2 min, respectively. SARS-CoV-2 RNA was found to be significantly more persistent than infectious SARS-CoV-2, indicating that the environmental detection of RNA alone does not substantiate risk of infection.