Impacts of domestication on the rhizobial mutualism of five legumes across a gradient of nitrogen-fertilisation
Impacts of domestication on the rhizobial mutualism of five legumes across a gradient of nitrogen-fertilisation
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DOI：
10.1007/s11104-023-06128-x
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发表时间：
2023-07
期刊：
影响因子：
4.9
通讯作者：
Niall Millar;J. Piovia‐Scott;S. Porter
中科院分区：
文献类型：
--
作者：
Niall Millar;J. Piovia‐Scott;S. Porter
目的本地化和作物育种改进了供人类使用的植物，但可能会对未经选择的性状产生意想不到的后果。在农作物中观察到了菌根互惠关系的破坏；可能是由于在肥沃的土壤中繁殖。尽管共生固氮很重要，但关于豆科植物-根瘤菌的互惠关系是否会被驯化和作物进化所破坏，人们知之甚少。通过5个温室试验，我们研究了鹰嘴豆、扁豆、豌豆、花生和大豆在不同氮肥水平下的共生特性，以确定野生和驯化品系之间的共生特性是否有明显的差异。结果在较低的氮素添加水平下，野生大豆比驯化大豆从根瘤菌中受益更多。与野生鹰嘴豆相比，鹰嘴豆品种在低氮条件下放弃了共生。鹰嘴豆品种比野生鹰嘴豆更能减少每个根瘤菌落形成单位(CFU)，而小扁豆品种比小扁豆减少CFU少。小扁豆、豌豆和花生品种与其野生近缘品种在根瘤菌效益和结瘤反应方面没有差异。结论驯化鹰嘴豆和野生鹰嘴豆与大豆在根瘤共生调控上存在明显差异，而小扁豆、豌豆和花生则不同。这表明，互惠共生的破坏--相互共生相互作用的程度的降低--可能是驯化的结果，但不是必然的。
AimsDomestication and crop breeding has improved plants for human use, but may have unintended consequences for traits that are not selected upon. Disruption in the mycorrhizal mutualism has been observed in crops; possibly due to breeding in fertilised soil. Little is known about whether the legume-rhizobia mutualism can be disrupted by domestication and crop evolution, despite the importance of symbiotic nitrogen fixation. We aimed to identify differences in mutualistic outcomes between five legume cultivars and their wild progenitors in terms of their reactions across varying nitrogen levels.MethodsWith five greenhouse experiments, we characterised symbiosis traits in chickpea, lentil, pea, peanut, and soybean across a gradient of N fertilisation to characterise whether symbiosis traits differ context specifically between wild and domesticated lines.ResultsAt lower levels of N addition, wild soybean benefited more from rhizobia than domesticated soybean. Chickpea cultivars abandoned symbiosis at lower N than wild chickpea accessions. Chickpea cultivars reduced per-nodule colony forming units (CFU) in response to N addition more than wild chickpeas, but lentil cultivars reduced CFU less than lentil accessions. The lentil, pea, and peanut cultivars did not differ from their wild relatives in rhizobial benefit, nor in nodulation response to N addition.ConclusionsDifferences in the regulation of the root nodule symbiosis are evident between domesticated and wild chickpea and soybean, but not lentil, pea, or peanut. This indicates that mutualism disruption—a decrease in the magnitude of the mutually symbiotic interaction—is a possible, but not necessary, consequence of domestication.