Effect of UV screens and preservatives on vitellogenin and choriogenin production in male medaka (Oryzias latipes)
Effect of UV screens and preservatives on vitellogenin and choriogenin production in male medaka (Oryzias latipes)
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DOI：
10.1016/s0300-483x(03)00340-8
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发表时间：
2003-12-15
期刊：
影响因子：
4.5
通讯作者：
Miyatake, K
中科院分区：
文献类型：
--
作者：
Inui, M;Adachi, T;Miyatake, K
紫外线 (UV) 屏蔽剂和防腐剂在化妆品和药品中的应用越来越广泛。在本研究中，我们使用雄性青鳉 (Oryzias latipes)，检查了紫外线屏蔽剂和防腐剂中 4-甲基亚苄基樟脑 (4-MBC)、甲氧基肉桂酸辛酯 (ONIC) 和对羟基苯甲酸丙酯 (PP) 的雌激素活性，并使用雄性青鳉 (Oryzias latipes) 来生产已知的卵黄蛋白原 (VTG) 和绒毛蛋白原 (CHG)。是雌激素反应性基因产物。首先，使用 VTG 酶联免疫吸附测定 (ELISA) 系统，我们确定了青鳉中由于暴露于 4-MBC、OMC 和 PP 而导致的 VTG 血浆浓度增加，并将该浓度与未处理的对照进行比较。接下来，我们发现与未处理的对照相比，由于暴露于 4-MBC、OMC 和 PP，肝脏中 VTG 亚型 VTG-1 和 VTG-2 以及 CHG 亚型 CHG-L 和 CHG-H 的 mRNA 表达水平增加。此外，我们还发现，与未处理的对照相比，由于暴露于 4-MBC、ONIC 和 PP，肝脏中性激素受体中雌激素受体 (ER) a. 的 mRNA 表达水平增加。在这项研究中，我们证明 4-MBC、ONIC 和 PP 对鱼类具有雌激素活性。 (C) 2003 Elsevier Ireland Ltd. 保留所有权利。
Ultra violet (UV) screens and preservatives are widely and increasingly used in cosmetics and pharmaceuticals. In the present study, we examined the estrogenicity of 4-methyl-benzylidene camphor (4-MBC), octyl-methoxycinnamate (ONIC), and propyl paraben (n-propyl-p-hydroxy-benzoate; PP), among UV screens and preservatives, using male medaka (Oryzias latipes), in regard to production of vitellogenin (VTG) and choriogenin (CHG) which are known to be estrogen-responsive gene products. First, using a VTG enzyme-linked immunosorbent assay (ELISA) system, we determined the increase in VTG plasma concentration in medaka due to exposure to 4-MBC, OMC, and PP, and compared this concentration to the non-treated control. Next, we found increases in mRNA expression levels of VTG subtypes VTG-1 and VTG-2, and CHG subtypes CHG-L and CHG-H, in liver due to exposure to 4-MBC, OMC, and PP compared to the non-treated control. In addition, we also found increased mRNA expression levels of estrogen receptor (ER) a., among sex hormone receptors in the liver, due to exposure to 4-MBC, ONIC, and PP compared to the non-treated control. In this study, we showed that 4-MBC, ONIC, and PP have estrogenic activity in fish. (C) 2003 Elsevier Ireland Ltd. All rights reserved.