Prokineticin 1 Protein Expression is a Useful New Prognostic Factor for Human Sporadic Colorectal Cancer
Prokineticin 1 Protein Expression is a Useful New Prognostic Factor for Human Sporadic Colorectal Cancer
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DOI：
10.1245/s10434-014-4150-3
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发表时间：
2015-05-01
影响因子：
3.7
通讯作者：
Yamaguchi, Akio
中科院分区：
文献类型：
--
作者：
Nakazawa, Toshiyuki;Goi, Takanori;Yamaguchi, Akio
血行转移被认为与血管生成生长因子密切相关，与结直肠癌预后密切相关。血管生成因子原激动素1(PROK1)已从内分泌细胞中克隆出来。然而，其在人类恶性肿瘤中的蛋白表达尚未见报道。本研究建立了抗PROK1的单抗，探讨了PROK1蛋白的表达与结直肠癌的关系。采用自制的人PROK1单抗免疫组织化学方法，检测了620例散发性结直肠癌组织中PROK1蛋白的表达，探讨其与临床病理因素、复发和生存率的关系及其预后意义。根据临床病理检查，浆膜侵犯、淋巴管侵犯、静脉侵犯、淋巴结转移、肝转移、血行转移和较高分期患者的PROK1阳性表达频率明显高于浆膜侵犯、淋巴管侵犯、静脉侵犯、肝转移、血行转移和较高分期的患者。PROK1表达阳性的患者复发率和预后明显较差。在Cox比例风险模型中，PROK1的表达是一个独立的预后因素，PROK1蛋白的表达首次被确认为结直肠癌的一个新的预后因素。
Hematogenous metastasis, regarded as closely related to angiogenic growth factors, is associated with colorectal cancer prognosis. The angiogenic growth factor prokineticin 1 (PROK1) has been cloned from endocrine cells. However, its protein expression in human malignant tumors has not been studied. The current study established the anti-PROK1 monoclonal antibody (mAb) and examined the relationship between the expression of PROK1 protein and human colorectal cancer.The expression of PROK1 protein was assessed in 620 resected sporadic colorectal cancer tissue samples by immunohistochemical staining with in-house-developed human PROK1 mAb to investigate the relationship of PROK1 expression to clinicopathologic factors, recurrence, and survival rate and to evaluate its prognostic significance.The expression of PROK1 protein was detected in 36 % (223/620) of human primary colorectal cancer lesions but no in the healthy mucosa adjacent to the colorectal cancer lesions. According to the clinicopathologic examinations, the frequency of positive PROK1 expression was significantly higher in cases with serosal invasion, lymphatic invasion, venous invasion, lymph node metastasis, liver metastasis, hematogenous metastasis, and higher stage disease. The recurrence rate and prognosis for patients with PROK1 expression-positive lesions were significantly worse. In the Cox proportional hazard model, PROK1 expression was an independent prognostic factor.The expression of PROK1 protein was identified for the first time as a new prognostic factor in colorectal cancer.