Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein
Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein
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通过 sCAR-PPA 融合蛋白检测暴露于甘露糖的骨髓性白血病细胞
DOI：
10.1007/s12185-009-0308-3
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发表时间：
2009-06-01
影响因子：
2.1
通讯作者：
Liu, Xiao Chuan
中科院分区：
文献类型：
--
作者：
Li, Gong Chu;Li, Na;Liu, Xiao Chuan
糖基化改变可能是恶性转化和癌症进展的标志。在所描述的工作中，特异性甘露糖结合凝集素，掌叶半夏凝集素（PPA），与柯萨奇-腺病毒受体（CAR）的胞外结构域基因融合，以产生可溶性CAR（sCAR）-PPA融合蛋白。sCAR-PPA增加了急性髓性白血病（AML）细胞系Kasumi-1和HL-60的腺病毒转导，表明PPA检测到一部分暴露甘露糖残基的AML细胞。然而，sCAR-PPA没有增加KG-1细胞的腺病毒感染，表明AML细胞的甘露糖暴露可能是细胞类型特异性的。此外，sCAR-PPA还能显著提高Ad-EGFP对慢性粒细胞白血病细胞株K562的感染效率。我们，在此，报告PPA承认一部分髓性白血病细胞显示甘露糖暴露表型。sCAR-PPA融合蛋白与腺病毒载体系统的结合可能为研究暴露甘露糖残基的髓系白血病细胞并进一步阐明这些细胞在白血病发展中的作用提供有用的工具。
Altered glycosylation may be a hallmark of malignant transformation and cancer progression. In the work described, a specific mannose-binding lectin,Pinellia pedatisectaagglutinin (PPA), was genetically fused with the extracellular domain of coxsackie-adenovirus receptor (CAR) to generate the soluble CAR (sCAR)-PPA fusion protein. The adenoviral transduction of acute myeloid leukemia (AML) cell lines Kasumi-1 and HL-60 was increased by sCAR-PPA, indicating that a fraction of AML cells exposing mannose residues was detected by PPA. However, sCAR-PPA did not increase the adenoviral infection of KG-1 cells, suggesting the mannose exposure of AML cells may be cell type specific. Furthermore, the infectious efficiency of Ad-EGFP in chronic myeloid leukemia cell line K562 was significantly increased by sCAR-PPA as well. We, herein, report that PPA recognized a fraction of myeloid leukemia cells showing mannose-exposing phenotype. The sCAR-PPA fusion protein combined with the adenoviral vector system may provide a useful tool for investigating myeloid leukemia cells exposing mannose residues and further elucidating the role of these cells in the leukemia development.