High throughput testing of the SV40 Large T antigen binding to cellular p53 identifies putative drugs for the treatment of SV40-related cancers.
High throughput testing of the SV40 Large T antigen binding to cellular p53 identifies putative drugs for the treatment of SV40-related cancers.
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对 SV40 大 T 抗原与细胞 p53 结合的高通量测试确定了用于治疗 SV40 相关癌症的推定药物。
DOI：
10.1016/s0042-6822(03)00547-6
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发表时间：
2003
期刊：
影响因子：
3.7
通讯作者：
Bocchetta,Maurizio
中科院分区：
文献类型：
--
作者：
Carbone,Michele;Rudzinski,Jennifer;Bocchetta,Maurizio
SV40与一些人类恶性肿瘤有关，并且这种病毒在间皮瘤和脑肿瘤中起致病作用的证据越来越多。主要的SV40癌蛋白是大肿瘤抗原（Tag）。关键的标签转化活性与其结合和抑制细胞p53的能力有关。在这项研究中，我们开发了一种有效的，高通量的，ELISA为基础的方法来研究标签p53在体外的相互作用。该测定允许我们筛选化学文库并鉴定与p53结合的标签的化学抑制剂。我们提出，我们的体外试验是一种有用的方法，以确定分子，可用作治疗剂的SV40相关的人类癌症的治疗。
SV40 has been linked to some human malignancies, and the evidence that this virus plays a causative role in mesothelioma and brain tumors is mounting. The major SV40 oncoprotein is the Large tumor antigen (Tag). A key Tag transforming activity is connected to its capability to bind and inactivate cellular p53. In this study we developed an effective, high throughput, ELISA-based method to study Tag-p53 interaction in vitro. This assay allowed us to screen a chemical library and to identify a chemical inhibitor of the Tag binding to p53. We propose that our in vitro assay is a useful method to identify molecules that may be used as therapeutic agents for the treatment of SV40-related human cancers.