Neutron-capture therapy of human cancer: in vitro results on the preparation of boron-labeled antibodies to carcinoembryonic antigen.
Neutron-capture therapy of human cancer: in vitro results on the preparation of boron-labeled antibodies to carcinoembryonic antigen.
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人类癌症的中子捕获疗法：制备硼标记癌胚抗原抗体的体外结果。
DOI：
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发表时间：
1982
影响因子：
11.1
通讯作者：
M. Hawthorne
中科院分区：
文献类型：
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作者：
E. Mizusawa;H. L. Dahlman;S. Bennett;D. Goldenberg;M. Hawthorne
通过用3 H2O（分别为0.1和5.0 Ci/ml）处理1-锂-2-苯基-1，2-二碳-闭合-十二硼烷（12）来制备2-苯基-1，2-二碳-闭合-[1- 3 H]十二硼烷（12）的两个样品。这些氚化苯基碳硼烷样品随后转化为适合于偶氮偶联反应的对-[1，2-二碳-闭合-[1- 3 H]十二硼烷（12）-2-基]苯重氮鎓离子（[3 H]DBD）的相应样品。两种氚化重氮离子样品与2-萘酚反应生成偶氮染料（λ = 1.98 × 10（4）M ~（-1）cm ~（-1），485 nm）。将吸光度与~ 3 H活性相关的实验证明，两种[~ 3 H]DBD源每摩尔氚化碳硼烷取代基具有3.81 × 10 ~（11）和2.45 × 10 ~（13）cpm的~ 3 H。将纯化的癌胚抗原抗体与[3 H]DBD偶联，并在广泛透析后，通过测量与已知蛋白质浓度相关的3 H活性来确定每个抗体分子的碳硼烷部分的平均数。进一步检查这些氚化碳硼烷标记的抗体亲和层析证明，硼标记并没有破坏他们的免疫反应性。偶氮偶联条件（反应物比例，pH值）与免疫反应性和抗体蛋白质回收率的相关性。
Two samples of 2-phenyl-1,2-dicarba-closo-[1-3H]dodecaborane(12) were prepared by treating 1-lithio-2-phenyl-1,2-dicarba-closo-dodecaborane(12) with 3H2O (0.1 and 5.0 Ci/ml, respectively). These tritiated phenylcarborane samples were subsequently converted to corresponding samples of p-[1,2-dicarba-closo-[1-3H]dodecaboran(12)-2-yl]benzenediazonium ion ([3H]DBD) suitable for azo-coupling reactions. Reaction of the two tritiated diazonium ion samples with 2-napthol resulted in the formation of an azo dye (epsilon = 1.98 X 10(4) M-1 cm-1 at 485 nm). Experiments relating absorbance to 3H activity proved the two [3H]DBD sources to have 3.81 X 10(11) and 2.45 X 10(13) cpm of 3H per mol of tritiated carborane substituent. Purified antibodies to carcinoembryonic antigen were coupled to the [3H]DBD and, after extensive dialysis, the average number of carborane moieties per antibody molecule was determined by measuring the 3H activity associated with a known protein concentration. Further examination of these tritiated carborane-labeled antibodies by affinity chromatography proved that boron labeling did not destroy their immunoreactivity. Correlations of azo-coupling conditions (reactant ratios, pH) with immunoreactivity and antibody protein recovery are presented.