TRANSIENT AND LOCALLY RESTRICTED EXPRESSION OF LAMININ-A CHAIN MESSENGER-RNA BY DEVELOPING EPITHELIAL-CELLS DURING KIDNEY ORGANOGENESIS
TRANSIENT AND LOCALLY RESTRICTED EXPRESSION OF LAMININ-A CHAIN MESSENGER-RNA BY DEVELOPING EPITHELIAL-CELLS DURING KIDNEY ORGANOGENESIS
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DOI：
10.1016/0092-8674(90)90748-4
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发表时间：
1990-01-26
期刊：
影响因子：
64.5
通讯作者：
EKBLOM, P
中科院分区：
文献类型：
--
作者：
EKBLOM, M;KLEIN, G;EKBLOM, P
已经描述了小鼠层粘连蛋白（一种基底膜蛋白）的三条多肽链A、B1和B2。我们研究了层粘连蛋白A，B1和B2 mRNA在发育中的小鼠肾脏中的表达。体外诱导肾间充质分化导致B1和B2链mRNA在发育第1天的表达增加。相反，A链mRNA的表达增加，在第2天，当上皮细胞极化开始。层粘连蛋白A mRNA和多肽在体内发育过程中也仅由上皮细胞表达。一些产生基底膜的极化细胞类型（内皮细胞，一些成人上皮细胞）缺乏A链mRNA和多肽，尽管它们表达B链。因此，具有400 kd A链的层粘连蛋白是在肾脏形态发生的特定位点出现的瞬时形式，而具有不同A链或没有A链的同种型具有更广泛的分布。
Three polypeptide chains, A, B1, and B2, have been described for mouse laminin, a basement membrane protein. We studied expression of laminin A, B1, and B2 mRNA in the developing mouse kidney. Induction of kidney mesenchyme differentiation in vitro led to an increased expression of B1 and B2 chain mRNA on day 1 of development. In contrast, expression of A chain mRNA increased on day 2, when epithelial cell polarization begins. Laminin A mRNA and polypeptide were expressed only by epithelia during in vivo development as well. Some polarized cell types producting basement membrane (endothelium, some adult epithelia) lacked the A chain mRNA and polypeptide, although they did express B chains. Laminin with the 400 kd A chain is therefore transient form appearing at specific sites of kidney morphogenesis, whereas isoforms with a different A chain or without it have a more widespread distribution.