Biological oxygen sensing via two-photon absorption by an Ir(III) complex using a femtosecond fiber laser
Biological oxygen sensing via two-photon absorption by an Ir(III) complex using a femtosecond fiber laser
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DOI：
10.7567/jjap.55.092401
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发表时间：
2016-08
影响因子：
1.5
通讯作者：
H. Moritomo;Akina Fujii;Yasutaka Suzuki;Toshitada Yoshihara;S. Tobita;J. Kawamata
中科院分区：
文献类型：
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作者：
H. Moritomo;Akina Fujii;Yasutaka Suzuki;Toshitada Yoshihara;S. Tobita;J. Kawamata
研究了磷光Ir（III）配合物（2,4-戊二酮-κ o2，κO4）双[2-（6-菲thridinyl-κ n）苯并[b]thien-3-yl-κ c]铱（BTPHSA）的近红外双光子吸收特性。该材料具有800 ~ 1200 nm的双光子吸收带，可被1030 nm飞秒掺钛蓝宝石和掺镱光纤激光器电子激发。利用BTPHSA对人胚胎肾293 （HEK293）细胞的氧浓度进行成像。这些结果证明了活组织的双光子氧传感通过易于操作的激发源。
Near-infrared two-photon absorption of the phosphorescent Ir(III) complex (2,4-pentanedionato-κO2,κO4)bis[2-(6-phenanthridinyl-κN)benzo[b]thien-3-yl-κC]iridium (BTPHSA) was characterized. It exhibited a 800–1200 nm two-photon absorption band, and thus could be electronically excited by 1030-nm femtosecond Ti:sapphire and Yb-doped fiber lasers. By using BTPHSA, oxygen concentrations in human embryonic kidney 293 (HEK293) cells were imaged. These results demonstrate two-photon oxygen sensing of live tissues via easily operable excitation sources.