ENHANCEMENT OF HUMORAL RESPONSE AGAINST HUMAN INFLUENZA VACCINE WITH THE SIMPLE SUBMICRON OIL/WATER EMULSION ADJUVANT MP59
ENHANCEMENT OF HUMORAL RESPONSE AGAINST HUMAN INFLUENZA VACCINE WITH THE SIMPLE SUBMICRON OIL/WATER EMULSION ADJUVANT MP59
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DOI：
10.1016/0264-410x(95)00089-j
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发表时间：
1995-11-01
期刊：
影响因子：
5.5
通讯作者：
VANNEST, G
中科院分区：
文献类型：
--
作者：
OTT, G;BARCHFELD, GL;VANNEST, G
人类流感亚单位疫苗对风险最大的非常年轻或老年人群都不是完全保护的。利用佐剂的脚趾来提高抗体效价是提高疫苗效力的一个有吸引力的选择。一系列角鲨烯/水乳剂由两亲性壁壁肽MTP-PE(钠N-acetyl-muramyl-L-alanyl-D-isoglutaminyl-L-alanyl-2-(1‘，2’-dipalmitoyl-sn-glycero-3‘phospho)ethylamide)或山梨醇油酸酯表面活性剂吐温80和司盘85的混合物稳定)作为流感疫苗的佐剂已被测试，流感疫苗与吐温/斯潘稳定乳剂的结合在广泛的一系列幼稚动物模型中导致了显著更高的疫苗抗体效价，使用亚微米乳液滴在确定佐剂活性方面具有重要意义。虽然佐剂活性不需要壁壁肽的存在，但仅包含低毒成分角鲨烯、吐温80和司潘85的直径200-300 nm的乳剂MF59已被证明可将效价提高5至250倍，即单独接种疫苗。
Human influenza subunit vaccines are not fully protective in either the very young or elderly populations where risk is greatest. The toe of an adjuvant to enhance antibody titer is an attractive option to increase vaccine efficacy. A series of squalene/H2O emulsions stabilized either by the amphipathic muramyl peptide MTP-PE (sodium N-acetyl-muramyl-L-alanyl-D-isoglutaminyl-L-alanyl-2-(1',2'-dipalmitoyl-sn-glycero-3'phospho)ethylamide) or by mixtures of the sorbitan oleate surfactants Tween 80 and Span 85 have been tested as adjuvants with influenza vaccine, Combination of influenza vaccine with the Tween/Span stabilized emulsions has resulted in significantly higher antibody titers to vaccine in an extensive series of naive animal models, The use of submicron emulsion droplets is significant in determination of adjuvant activity, while the presence of the muramyl peptide is not required for adjuvant activity, The 200-300 nm diameter emulsion formulation MF59 containing only the low toxicity components squalene, Tween 80 and Span 85 has been shown to enhance titers from 5 to 250 times that achievable, vith vaccine alone.