Biophysical studies of lipid nanodomains using different physical characterization techniques
Biophysical studies of lipid nanodomains using different physical characterization techniques
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DOI：
10.1016/j.bpj.2023.01.024
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发表时间：
2023-03-21
影响因子：
3.4
通讯作者：
Katsaras，John
中科院分区：
文献类型：
--
作者：
Kinnun，Jacob J.;Scott，Haden L.;Katsaras，John
在过去的50年里，功能类脂结构域存在的证据一直在稳步积累。尽管功能脂类结构域，也被称为“脂筏”的概念现在被广泛接受，但情况并不总是如此。这种围绕脂类结构域的模糊性可能部分归因于它们是高度动态的纳米结构。由于最常用的技术对微尺度结构特征很敏感，因此，花了一些时间才就它们的存在达成共识也就不足为奇了。在这篇综述文章中，我们将讨论使用对纳米结构特征(即中子散射、核磁共振和Förster共振能量转移)固有敏感的技术的研究。我们还将提到未来可能有用的技术(即冷冻电子显微镜、液滴界面双层、非弹性x射线散射和中子反射术)，这些技术可以加深我们对纳米脂类结构域不同和独特的物理化学性质的理解。
For the past 50 years, evidence for the existence of functional lipid domains has been steadily accumulating. Although the notion of functional lipid domains, also known as "lipid rafts," is now widely accepted, this was not always the case. This ambiguity surrounding lipid domains could be partly attributed to the fact that they are highly dynamic, nanoscopic structures. Since most commonly used techniques are sensitive to microscale structural features, it is therefore, not surprising that it took some time to reach a consensus regarding their existence. In this review article, we will discuss studies that have used techniques that are inherently sensitive to nanoscopic structural features (i.e., neutron scatting, nuclear magnetic resonance, and Förster resonance energy transfer). We will also mention techniques that may be of use in the future (i.e., cryoelectron microscopy, droplet interface bilayers, inelastic x-ray scattering, and neutron reflectometry), which can further our understanding of the different and unique physicochemical properties of nanoscopic lipid domains.