A HiPAD Integrated with rGO/MWCNTs Nano-Circuit Heater for Visual Point-of-Care Testing of SARS-CoV-2
A HiPAD Integrated with rGO/MWCNTs Nano-Circuit Heater for Visual Point-of-Care Testing of SARS-CoV-2
复制标题
与 rGO/MWCNT 纳米电路加热器集成的 HiPAD，用于 SARS-CoV-2 的视觉护理点测试
DOI：
10.1002/adfm.202100801
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发表时间：
2021-04-20
影响因子：
19
通讯作者：
Ding, Xianting
中科院分区：
文献类型：
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作者：
Li, Sijie;Huang, Shiyi;Ding, Xianting
如今，核酸点检设备进一步小型化和集成化的主要障碍是缺乏低成本和高性能的加热材料来支持可靠的核酸扩增。在这里，我们开发了一种集成在巧妙的纸基加热器中的还原氧化石墨烯杂化多壁碳纳米管纳米电路，并将其集成到纸基分析装置(称为HiPad)中。由严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)引发的冠状病毒病2019年(新冠肺炎)大流行仍在全球肆虐。作为概念验证，利用HiPad通过有色环介导的等温扩增反应对SARS-CoV-2N基因进行了可视化检测。这款几美元的HiPad的检测性能可以与传统的数千美元的核酸放大器相媲美。检测范围为25-2.5×10(10)拷贝毫升(-1)，45min。该方法的检测下限为25拷贝(-1)，是常规实时荧光PCR仪器中1000拷贝(-1)的灵敏度的40倍。这种一次性纸质芯片还可以通过方便的焚烧避免新冠肺炎的潜在二次传播，确保生物安全。HiPad或易于扩展的M-HiPad(用于多重检测)在资源有限的环境中具有巨大的病原体诊断潜力。
Nowadays, the main obstacle for further miniaturization and integration of nucleic acids point-of-care testing devices is the lack of low-cost and high-performance heating materials for supporting reliable nucleic acids amplification. Herein, reduced graphene oxide hybridized multi-walled carbon nanotubes nano-circuit integrated into an ingenious paper-based heater is developed, which is integrated into a paper-based analytical device (named HiPAD). The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still raging across the world. As a proof of concept, the HiPAD is utilized to visually detect the SARS-CoV-2 N gene using colored loop-mediated isothermal amplification reaction. This HiPAD costing a few dollars has comparable detection performance to traditional nucleic acids amplifier costing thousands of dollars. The detection range is from 25 to 2.5 x 10(10) copies mL(-1) in 45 min. The detection limit of 25 copies mL(-1) is 40 times more sensitive than 1000 copies mL(-1) in conventional real-time PCR instruments. The disposable paper-based chip could also avoid potential secondary transmission of COVID-19 by convenient incineration to guarantee biosafety. The HiPAD or easily expanded M-HiPAD (for multiplex detection) has great potential for pathogen diagnostics in resource-limited settings.