High-performance affinity beads for identifying drug receptors
High-performance affinity beads for identifying drug receptors
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DOI：
10.1038/78496
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发表时间：
2000-08-01
影响因子：
46.9
通讯作者：
Handa, H
中科院分区：
文献类型：
--
作者：
Shimizu, N;Sugimoto, K;Handa, H
我们开发了一种利用新型乳胶珠通过亲和纯化快速鉴定药物受体的方法。由甲基丙烯酸缩水甘油酯(GMA)和具有GMA聚合物表面的苯乙烯共聚物核组成的微珠，最大限度地减少了非特异性蛋白质结合，并最大限度地提高了净化效率。我们用FK506-偶联珠有效地纯化了FK506结合蛋白，证明了它们的性能，并发现与以前的方法相比，所需的材料量显著减少。利用胶乳小球，我们确定了氧化还原相关因子Ref-1作为抗核因子-kappaB药物E3330的靶蛋白，证明了一类新的抗核因子-kappaB药物受体的存在。我们的结果表明，乳胶珠可以为药物受体的鉴定和分析提供一种工具，因此在药物开发中应该是有用的。
We have developed a method using novel latex beads for rapid identification of drug receptors using affinity purification. Composed of a glycidylmethacrylate (GMA) and styrene copolymer core with a GMA polymer surface, the beads minimize nonspecific protein binding and maximize purification efficiency. We demonstrated their performance by efficiently purifying FK506-binding protein using FK506-conjugated beads, and found that the amount of material needed was significantly reduced compared with previous methods. Using the latex beads, we identified a redox-related factor, Ref-1, as a target protein of an anti-NF-kappa B drug, E3330, demonstrating the existence of a new class of receptors of anti-NF-kappa B drugs. Our results suggest that the latex beads could provide a tool for the identification and analysis of drug receptors and should therefore be useful in drug development.