Population Genetics of Konosirus punctatus in Chinese Coastal Waters Inferred From Two mtDNA Genes (COI and Cytb)
Population Genetics of Konosirus punctatus in Chinese Coastal Waters Inferred From Two mtDNA Genes (COI and Cytb)
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从两个mtDNA基因（COI和Cytb）推断中国沿海马尾Konosirus punctatus的种群遗传学
DOI：
10.3389/fmars.2020.00534
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发表时间：
2020-06-30
影响因子：
3.7
通讯作者：
Lu, Zhenming
中科院分区：
文献类型：
--
作者：
Liu, Bingjian;Zhang, Kun;Lu, Zhenming
斑点金鱼生活在中国近海具有重要商业价值的地区。利用两个线粒体DNA基因对分布于中国沿海的12个种群进行了分析。分别从242个和259个个体中扩增出细胞色素氧化酶亚基I(COI)和细胞色素b(Cytb)基因，并用999和415个碱基的片段进行分析。COI和Cytb单倍型多样性较高，核苷酸多样性较低。COI mtDNA基因具有较高的群体内变异和较低的群体间变异，而Cytb则表现出较高的群体间变异和较低的群体内变异。COI和Cytb的F-ST值显示，除中山种群的Cytb基因具有较高的FST值外，其余群体间的遗传分化程度较低。构建的系统发育树和单倍型网络树显示出单一的谱系，而cytb基因数据显示中山群体在一个群体内存在较大的差异水平。本研究对斑点石斑鱼的遗传结构有了一定的了解，对今后的保护工作具有一定的参考价值。
Konosirus punctatus inhabits areas of significant commercial importance in Chinese coastal waters. Twelve populations distributed all along the Chinese coast were analyzed using two mtDNA genes. The cytochrome oxidase subunit I (COI) and cytochrome b (Cytb) genes were amplified from 242 and 259 individuals, respectively, and fragments of 999 and 415 bp sequence in length were used for analysis. COI and Cytb denoted a high haplotype diversity and low nucleotide diversity. The COI mtDNA gene revealed a high molecular variance within populations and low variance among populations, whereas Cytb showed a high variance among populations and low within populations. F-ST values obtained for COI and Cytb showed low genetic differentiation among the populations, except for the Zhongshan population where the Cytb gene showed high FST values. The constructed phylogenetic tree and haplotype network tree showed a single lineage, however, Cytb gene data showed that the Zhongshan population has a greater difference level within a population. The present investigation contributed to existing knowledge of the genetic structure of K. punctatus and can be useful for its future conservation.