Metagenomics and CAZyme Discovery
Metagenomics and CAZyme Discovery
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DOI：
10.1007/978-1-4939-6899-2_20
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发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Pope, Phillip B.
中科院分区：
文献类型：
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作者：
Kunath, Benoit J.;Bremges, Andreas;Pope, Phillip B.
微生物在调节生物地球化学循环中起主要作用，并且是具有生物技术应用的酶的宝贵来源，例如碳水化合物活性酶（CAZymes）。然而，无法使用常见的培养依赖性技术培养自然生态系统中存在的大多数微生物，限制了获得潜在的新型纤维素分解细菌和有益酶。宏基因组学等基于分子的不依赖培养的方法的发展使研究人员能够直接从环境样品中研究微生物群落，并提供了一个平台，从中可以获得感兴趣的酶。我们概述了所需的关键方法学阶段，并描述了目前用于CAZyme发现的宏基因组项目的具体协议。
Microorganisms play a primary role in regulating biogeochemical cycles and are a valuable source of enzymes that have biotechnological applications, such as carbohydrate-active enzymes (CAZymes). However, the inability to culture the majority of microorganisms that exist in natural ecosystems using common culture-dependent techniques restricts access to potentially novel cellulolytic bacteria and beneficial enzymes. The development of molecular-based culture-independent methods such as metagenomics enables researchers to study microbial communities directly from environmental samples, and presents a platform from which enzymes of interest can be sourced. We outline key methodological stages that are required as well as describe specific protocols that are currently used for metagenomic projects dedicated to CAZyme discovery.