ACTIVITIES OF LIPASES AND CARNITINE PALMITOYLTRANSFERASE IN MUSCLES FROM VERTEBRATES AND INVERTEBRATES
ACTIVITIES OF LIPASES AND CARNITINE PALMITOYLTRANSFERASE IN MUSCLES FROM VERTEBRATES AND INVERTEBRATES
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DOI：
10.1042/bj1300697
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发表时间：
1972-01-01
影响因子：
4.1
通讯作者：
NEWSHOLME, EA
中科院分区：
文献类型：
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作者：
CRABTREE, B;NEWSHOLME, EA
1.三，二和单甘油酯脂肪酶和肉毒碱棕榈酰转移酶的活性测定在各种肌肉匀浆。这些活动被用来估计利用率的甘油酯和脂肪酸的肌肉。在肌肉的脂肪利用率的估计可以与计算的完整的肌肉从这样的信息，如O2摄取率，有合理的协议之间的估计和计算的速率。2.在所有的肌肉调查的最大速率的水解甘油酯增加的顺序甘油三酯，甘油二酯，甘油单酯。甘油二酯脂肪酶的活性在昆虫如蝗虫、水蝽和一些蛾的飞行肌中最高，而在苍蝇、蜜蜂和黄蜂的飞行肌中最低。这些结果与甘油二酯作为某些昆虫飞行肌肉的燃料的利用是一致的。3.在脊椎动物和无脊椎动物的许多肌肉中，甘油激酶的活性与脂肪酶的活性相似。它的结论是，在这些肌肉中的甘油激酶的代谢作用是去除脂肪分解过程中产生的甘油。然而，在一些昆虫的飞行肌肉中，甘油激酶的活性远大于脂肪酶的活性，这表明甘油激酶在这些肌肉中的不同作用。
1. The activities of tri-, di- and mono-glyceride lipase and carnitine palmitoyltransferase were measured in homogenates of a variety of muscles. These activities were used to estimate the rate of utilization of glycerides and fatty acids by muscle. In muscles whose estimated rates of fat utilization can be compared with rates calculated for the intact muscle from such information as O2uptake, there is reasonable agreement between the estimated and calculated rates. 2. In all muscles investigated the maximum rates of hydrolysis of glycerides increase in the order triglyceride, diglyceride, monoglyceride. The activity of diglyceride lipase is highest in the flight muscles of insects such as the locust, waterbug and some moths and is lowest in the flight muscles of flies, bees and the wasp. These results are consistent with the utilization of diglyceride as a fuel for some insect flight muscles. 3. In many muscles from both vertebrates and invertebrates the activity of glycerol kinase is similar to that of lipase. It is concluded that in these muscles the metabolic role of glycerol kinase is the removal of glycerol produced during lipolysis. However, in some insect flight muscles the activity of glycerol kinase is much greater than that of lipase, which suggests a different role for glycerol kinase in these muscles.