Comparison of the signaling pathways of wing dimorphism regulated by biotic and abiotic stress in the brown planthopper
Comparison of the signaling pathways of wing dimorphism regulated by biotic and abiotic stress in the brown planthopper
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褐飞虱生物与非生物胁迫调控翅二态性信号通路比较
DOI：
10.1111/1744-7917.13149
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发表时间：
2022-12-07
期刊：
影响因子：
4
通讯作者：
Zhang，Wen-Qing
中科院分区：
文献类型：
--
作者：
Chen，Jing-Xiang;Li，Wan-Xue;Zhang，Wen-Qing
翅膀多态性是多种昆虫普遍存在的一种进化特征，为研究昆虫传播的进化意义提供了模型系统。褐飞虱（Nilaparvata lugens）可以在生物和非生物胁迫下改变其翅膀形态。然而，这两种应激是否诱导了不同的信号通路仍然是未知的。在此，我们通过加权基因共表达网络分析（WGCNA）筛选了一些候选基因，发现鸟氨酸脱羧酶（NlODC）是合成多胺的关键酶，与BPH的翅膀分化有关，主要响应非生物应激刺激。我们通过转录组比较分析了两种胁迫下差异表达基因的京都基因百科全书和基因组富集途径，发现生物胁迫主要影响胰岛素相关信号通路，而非生物胁迫主要影响激素相关信号通路。此外，我们发现胰岛素受体1 （NlInR1）可能通过响应生物和非生物胁迫来调节BPH的翅膀分化，但NlInR2仅响应生物胁迫。同样，与幼激素降解和NlODC相关的幼激素环氧化物水解酶可能主要通过非生物胁迫调节翅膀分化。提出了一种基于基因和胁迫调控褐飞虱翅膀二态性的模型。这些发现揭示了生物和非生物胁迫诱导BPH中翅膀多态性的综合分子机制。
Wing polymorphism is an evolutionary trait that is widely present in various insects and provides a model system for studying the evolutionary significance of insect dispersal. The brown planthopper (BPH, Nilaparvata lugens) can alter its wing morphs under biotic and abiotic stress. However, whether differential signaling pathways are induced by the 2 types of stress remain largely unknown. Here, we screened a number of candidate genes through weighted gene co‐expression network analysis (WGCNA) and found that ornithine decarboxylase (NlODC), a key enzyme in the synthesis of polyamines, was associated with wing differentiation in BPH and mainly responded to abiotic stress stimuli. We analyzed the Kyoto Encyclopedia of Genes and Genomes enrichment pathways of differentially expressed genes under the 2 stresses by transcriptomic comparison, and found that biotic stress mainly influenced insulin‐related signaling pathways while abiotic stress mainly influenced hormone‐related pathways. Moreover, we found that insulin receptor 1 (NlInR1) may regulate wing differentiation of BPH by responding to both biotic and abiotic stress, but NlInR2 only responded to biotic stress. Similarly, the juvenile hormone epoxide hydrolase associated with juvenile hormone degradation and NlODC may regulate wing differentiation mainly through abiotic stress. A model based on the genes and stresses to modulate the wing dimorphism of BPH was proposed. These findings present a comprehensive molecular mechanism for wing polymorphism in BPH induced by biotic and abiotic stress.